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Titolo:
Isoform patterns of chitinase and beta-1,3-glucanase in maturing corn kernels (Zea mays L.) associated with Aspergillus flavus milk stage infection
Autore:
Ji, C; Norton, RA; Wicklow, DT; Dowd, PF;
Indirizzi:
Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 is, Dept Crop Sci, Urbana, IL 61801 USA USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA USDA ARS PeoriaIL USA 61604 l Ctr Agr Utilizat Res, Peoria, IL 61604 USA
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 2, volume: 48, anno: 2000,
pagine: 507 - 511
SICI:
0021-8561(200002)48:2<507:IPOCAB>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
PATHOGENESIS-RELATED PROTEINS; SYSTEMIC ACQUIRED-RESISTANCE; ANTIFUNGAL ACTIVITY; MAIZE; ELECTROPHORESIS; INDUCTION; CUCUMBER; VIRUS;
Keywords:
isoform; beta-1,3-glucanase; chitinase; corn kernels; Aspergillus flavus; aflatoxin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ji, C Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 pt Crop Sci, Urbana, IL 61801 USA
Citazione:
C. Ji et al., "Isoform patterns of chitinase and beta-1,3-glucanase in maturing corn kernels (Zea mays L.) associated with Aspergillus flavus milk stage infection", J AGR FOOD, 48(2), 2000, pp. 507-511

Abstract

Isoform patterns of chitinase and beta-1,3-glucanase of maturing kernels of yellow dent corn (Pioneer 3394) infected with Aspergillus flavus at the milk stage were investigated through polyacrylamide gel electrophoresis (PAGE). Proteins on the sodium dodecyl sulfate (SDS) gel with an apparent se molecular mass range of 23-46 kDa were differentially present in the kernels infected with both aflatoxin-producing and non-aflatoxin-producing strains of A. flavus. From in-gel (native PAGE) enzyme activity assays, three bandscorresponding to chitinase isoforms and two bands corresponding to beta-1,3-glucanase isoforms were detected in the infected kernels. One chitinase isoform of 29 kDa was present only in the infected kernels, and another one of 28 kDa was present in both infected and noninfected kernels. They were judged to be acidic on the basis of their migration on an acrylamide isoelectric focusing (IEF) gel. For the beta-1,3-glucanase, one isoform of 35 kDa was present in both infected and noninfected kernels, but another one, a 33kDa isoform, was present only in the infected kernels. Both acidic and basic beta-1,3-glucanase isoforms were detected in the IEF gel. The results ofthis study are the first to demonstrate patterns of enhanced or inducible proteins in maturing corn kernels in response to A. flavus infection at themilk stage. The results also indicate that only particular isoforms of thetwo hydrolytic enzymes are involved in the maturing corn kernels infected at the milk stage with A. flavus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:54:56