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Titolo:
Tumour necrosis factor alpha mRNA expression in early multiple sclerosis lesions: Correlation with demyelinating activity and oligodendrocyte pathology
Autore:
Bitsch, A; Kuhlmann, T; Da Costa, C; Bunkowski, S; Polak, T; Bruck, W;
Indirizzi:
Inst Neuropathol, D-13353 Berlin, Germany Inst Neuropathol Berlin Germany D-13353 opathol, D-13353 Berlin, Germany Univ Gottingen, Abt Neurol, Neurol Klin & Poliklin, D-3400 Gottingen, Germany Univ Gottingen Gottingen Germany D-3400 iklin, D-3400 Gottingen, Germany Univ Gottingen, Inst Neuropathol, D-3400 Gottingen, Germany Univ Gottingen Gottingen Germany D-3400 athol, D-3400 Gottingen, Germany
Titolo Testata:
GLIA
fascicolo: 4, volume: 29, anno: 2000,
pagine: 366 - 375
SICI:
0894-1491(20000215)29:4<366:TNFAME>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; MESSENGER-RNA EXPRESSION; ADHESION MOLECULE; CELL-DEATH; IN-SITU; CYTOKINE; APOPTOSIS; LYMPHOTOXIN; RECEPTOR;
Keywords:
apoptosis; demyelination; macrophages; microglia; T lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Bruck, W Inst Neuropathol, Campus Virchow Klinikum,Augustenburger Pl 1, D-13353 Berlin, Germany Inst Neuropathol Campus Virchow Klinikum,Augustenburger Pl 1 Berlin Germany D-13353
Citazione:
A. Bitsch et al., "Tumour necrosis factor alpha mRNA expression in early multiple sclerosis lesions: Correlation with demyelinating activity and oligodendrocyte pathology", GLIA, 29(4), 2000, pp. 366-375

Abstract

The precise role of tumour necrosis factor alpha (TNF alpha) in multiple sclerosis (MS) is still controversial. Most findings from the animal model experimental allergic encephalomyelitis have yet to be confirmed in multiplesclerosis. The aim of this study was to define the significance of TNF alpha with respect to the hallmark of MS, that is demyelination. Therefore, 78lesion areas from diagnostic brain biopsies of 32 patients were analysed. Lesion demyelinating activity was classified by the presence of myelin degradation products in macrophages and macrophage activation markers. Nonradioactive in situ hybridisation tvas carried out to detect TNF alpha mRNA expressing cells. DNA fragmentation was visualised by TdT-mediated X-dUTP nick end labeling. A significantly higher number of cells expressed TNF alpha mRNA in active demyelinating lesions than in inactive or remyelinating lesions irrespective of the extent of the inflammatory infiltrate. TNF alpha mRNAexpression correlated with the appearance of DNA fragmentation in T lymphocytes and oligodendrocytes within the lesions. In the periplaque white matter, expression of TNF alpha mRNA negatively correlated with oligodendrocytenumbers. These data support previous findings from animal models and in vitro experiments. Although not proving, the current study strongly suggests a pathogenic role of TNF alpha in demyelination in human multiple sclerosisand gives further support for TNF alpha-directed therapeutic strategies. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:09:47