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Titolo:
In vitro blastocyst formation of human oocytes obtained from unstimulated and stimulated cycles after vitrification at various maturational stages
Autore:
Chung, HM; Hong, SW; Lim, JM; Lee, SH; Cha, WT; Ko, JJ; Han, SY; Choi, DH; Cha, KY;
Indirizzi:
Pochon CHA Univ, Infertil Med Ctr, CHA Gen Hosp, Coll Med, Seoul 135081, South Korea Pochon CHA Univ Seoul South Korea 135081 Med, Seoul 135081, South Korea
Titolo Testata:
FERTILITY AND STERILITY
fascicolo: 3, volume: 73, anno: 2000,
pagine: 545 - 551
SICI:
0015-0282(200003)73:3<545:IVBFOH>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTRACYTOPLASMIC SPERM INJECTION; BOVINE OOCYTES; IN-VITRO; INVITRO FERTILIZATION; CRYOPRESERVATION; PREGNANCY; BIRTH; FROZEN;
Keywords:
human; oocyte; vitrification; in vitro maturation; chromosomal normality;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Chung, HM Pochon CHA Univ, Infertil Med Ctr, CHA Gen Hosp, Coll Med, 606-5Yeoksam 1Dong, Seoul 135081, South Korea Pochon CHA Univ 606-5 Yeoksam 1 Dong Seoul South Korea 135081 a
Citazione:
H.M. Chung et al., "In vitro blastocyst formation of human oocytes obtained from unstimulated and stimulated cycles after vitrification at various maturational stages", FERT STERIL, 73(3), 2000, pp. 545-551

Abstract

Objective: To evaluate the developmental competence and chromosomal normality of oocytes vitrified at various times after maturation culture. Design: In vitro model study. Setting: A university-affiliated hospital. Patient(s): Unstimulated women who underwent cesarean section or oophorectomy and infertile women who underwent a long protocol of GnRH stimulation. Intervention(s): Retrieved oocytes were vitrified at 0 or 48 hours after culture in unstimulated cycles and at 0, 8-15, or 24-28 hours after culture in stimulated cycles. Main Outcome Measure(s): Postthaw morphologic normality, maturation, fertilization, cleavage, blastocyst formation, and chromosome number. Result(s): In the 53 oocytes that were obtained from unstimulated cycles, no statistically significant differences were found in rates of morphologicnormality (range, 56%-63%) or fertilization (range, 31%-37%) according to the time of vitrification. In the SCI oocytes that were obtained from stimulated cycles, more of those that were vitrified at 24-28 hours were morphologically normal than those that were vitrified at 0 or 8-15 hours. Regardless of these differences, high cleavage rates (83%-100%) were obtained that did not differ significantly among the treatment groups. In both cycles, 20%-43% of cleaved oocytes developed to the blastocyst stage by 6 days after IVF. All the karyotyped blastocysts, three from unstimulated cycles and four from stimulated cycles, had a normal number of chromosomes. Conclusion(s): Vitrified and thawed oocytes from unstimulated or stimulated cycles developed to the blastocyst stage, regardless of when vitrification occurred; the number of chromosomes in the blastocysts was normal. (C) 2000 by American Society for Reproductive Medicine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 12:41:58