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Titolo:
Biosynthesis of dystroglycan: processing of a precursor propeptide
Autore:
Holt, KH; Crosbie, RH; Venzke, DP; Campbell, KP;
Indirizzi:
Univ Iowa, Coll Med, Howard Hughes Med Inst, Dept Physiol & Biophys, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 ysiol & Biophys, Iowa City, IA 52242 USA Univ Iowa, Coll Med, Howard Hughes Med Inst, Dept Neurol, Iowa City, IA 52242 USA Univ Iowa Iowa City IA USA 52242 st, Dept Neurol, Iowa City, IA 52242 USA
Titolo Testata:
FEBS LETTERS
fascicolo: 1, volume: 468, anno: 2000,
pagine: 79 - 83
SICI:
0014-5793(20000218)468:1<79:BODPOA>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYSTROPHIN-GLYCOPROTEIN COMPLEX; CONGENITAL MUSCULAR-DYSTROPHY; ALPHA-DYSTROGLYCAN; CARDIOMYOPATHIC HAMSTER; LAMININ ALPHA-2-CHAIN; EXTRACELLULAR-MATRIX; SARCOGLYCAN COMPLEX; BETA-DYSTROGLYCAN; PERIPHERAL-NERVE; SKELETAL-MUSCLE;
Keywords:
dystroglycan; glycosylation; Chinese hamster ovary cell; dystrophin-glycoprotein complex;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Campbell, KP Univ Iowa, Coll Med, Howard Hughes Med Inst, Dept Physiol & Biophys, 400 Eckstein Med Res Bldg, Iowa City, IA 52242 USA Univ Iowa 400 Eckstein Med Res Bldg Iowa City IA USA 52242 SA
Citazione:
K.H. Holt et al., "Biosynthesis of dystroglycan: processing of a precursor propeptide", FEBS LETTER, 468(1), 2000, pp. 79-83

Abstract

Dystroglycan is a cytoskeleton-linked extracellular matrix receptor expressed in many cell types. Dystroglycan is composed of alpha- and beta-subunits which are encoded by a single mRNA. Using a heterologous mammalian expression system, we provide the first biochemical evidence of the alpha/beta-dystroglycan precursor propeptide prior to enzymatic cleavage. This 160 kDa dystroglycan propeptide is processed into alpha- and beta-dystroglycan (120 kDa and 43 kDa, respectively), We also demonstrate that the precursor propeptide is glycosylated and that blockade of asparagine-linked (N-linked) glycosylation did not prevent the cleavage of the dystroglycan precursor peptide. However, inhibition of N-linked glycosylation results in aberrant trafficking of the alpha- and beta-dystroglycan subunits to the plasma membrane. Thus, dystroglycan is synthesized as a precursor propeptide that is post-translationally cleaved and differentially glycosylated to yield alpha- and beta-dystroglycan. (C) 2000 Federation of European Biochemical Societies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:18:00