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Titolo:
RECOMBINANT HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR INCREASES CIRCULATING CD34 POSITIVE CELLS IN PATIENTS WITH AIDS
Autore:
NIELSEN SD; DAMLARSEN S; NIELSEN C; WANTZIN P; MATHIESEN L; HANSEN JES;
Indirizzi:
UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT INFECT DIS DK-2650 HVIDOVRE DENMARK UNIV COPENHAGEN,HVIDOVRE HOSP,DEPT INFECT DIS DK-2650 HVIDOVRE DENMARK STATENS SERUM INST,RETROVIRUS LAB DK-2300 COPENHAGEN DENMARK UNIV COPENHAGEN,HVIDOVRE HOSP,BLOOD BANK DK-2650 HVIDOVRE DENMARK
Titolo Testata:
Annals of hematology
fascicolo: 5, volume: 74, anno: 1997,
pagine: 215 - 220
SICI:
0939-5555(1997)74:5<215:RHGFIC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; HEMATOPOIETIC PROGENITOR CELLS; PERIPHERAL-BLOOD LYMPHOCYTES; BONE-MARROW; GENE-THERAPY; TYPE-1; QUANTITATION; REPLICATION; ZIDOVUDINE; INFECTION;
Keywords:
CD34; HIV; GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF); HIV-1 RNA-PCR; GENE THERAPY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
S.D. Nielsen et al., "RECOMBINANT HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR INCREASES CIRCULATING CD34 POSITIVE CELLS IN PATIENTS WITH AIDS", Annals of hematology, 74(5), 1997, pp. 215-220

Abstract

In a gene therapy-based treatment of AIDS, it would be desirable to have as many transduced target cells as possible. A limiting factor is the number of target cells. In this study, we investigated whether it was possible to increase the absolute number of one possible target cell, i.e., the circulating hematopoietic progenitor cells (CD34 cells) in patients with AIDS, using the recombinant human granulocyte colony-stimulating factor (G-CSF). Eight patients with AIDS were treated withG-CSF for neutropenia (<1.0 x 10(9)/l). Treatment consisted of daily subcutaneous injections with 300 mu g G-CSF for 3-5 consecutive days. Within 5 days of initiation of G-CSF therapy, an increase in the absolute neutrophil count (ANC) was seen in all patients. There was a median increase in ANC from 0.4 to 3.4 x 10(9)/l. In addition, G-CSF treatment significantly increased the absolute number of CD34 cells. The median increase in CD34 cells was from 0.8 to 2.2 x 10(6)/l. Finally, using a highly sensitive HIV-1 RNA PCR, we found that treatment of AIDS patients with G-CSF did not lead to enhanced HIV replication. These observations indicate that G-CSF may be used to mobilize CD34 cells in patients with AIDS, e.g., for a gene therapy protocol.

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Documento generato il 24/11/20 alle ore 14:11:35