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Titolo:
Musashi1: An evolutionally conserved marker for CNS progenitor cells including neural stem cells
Autore:
Kaneko, Y; Sakakibara, S; Imai, T; Suzuki, A; Nakamura, Y; Sawamoto, K; Ogawa, Y; Toyama, Y; Miyata, T; Okano, H;
Indirizzi:
Osaka Univ, Grad Sch Med, Biomed Res Ctr, Dept Neurosci,Div Neuroanat, Suita, Osaka 565, Japan Osaka Univ Suita Osaka Japan 565 i,Div Neuroanat, Suita, Osaka 565, Japan Univ Tsukuba, Inst Basic Med Sci, Dept Anat, Tsukuba, Ibaraki 305, Japan Univ Tsukuba Tsukuba Ibaraki Japan 305 Anat, Tsukuba, Ibaraki 305, Japan Japan Sci & Technol Corp, Core Res Evolut Sci & Technol, Tokyo, Japan Japan Sci & Technol Corp Tokyo Japan Evolut Sci & Technol, Tokyo, Japan Keio Univ, Sch Med, Dept Orthopaed Surg, Tokyo, Japan Keio Univ Tokyo Japan Univ, Sch Med, Dept Orthopaed Surg, Tokyo, Japan
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 1-2, volume: 22, anno: 2000,
pagine: 139 - 153
SICI:
0378-5866(200001/04)22:1-2<139:MAECMF>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADULT MAMMALIAN BRAIN; CENTRAL-NERVOUS-SYSTEM; ZONE CELLS; EMBRYONIC FOREBRAIN; SUBVENTRICULAR ZONE; NEURONAL MIGRATION; SUBEPENDYMAL CELLS; CEREBELLAR CORTEX; MOUSE-BRAIN; PROTEIN;
Keywords:
Musashi1; Musashi2; monoclonal antibody; neural progenitor cell; neural stem cell; neurosphere; astrocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Okano, H Osaka Univ, Grad Sch Med, Dept Neuroanat D12, 2-2 Yamadaoka, Osaka 5650871, Japan Osaka Univ 2-2 Yamadaoka Osaka Japan 5650871 saka 5650871, Japan
Citazione:
Y. Kaneko et al., "Musashi1: An evolutionally conserved marker for CNS progenitor cells including neural stem cells", DEV NEUROSC, 22(1-2), 2000, pp. 139-153

Abstract

In situ detection of neural progenitor cells including stem-like cells is essential for studying the basic mechanisms of the generation of cellular diversity in the CNS, upon which therapeutic treatments for CNS injuries, degenerative diseases, and brain tumors may be based. We have generated rat monoclonal antibodies (Mab 14H1 and 14B8) that recognize an RNA-binding protein Musashi1, but not a Musashi1-related protein, Musashi2. The amino acid sequences at the epitope sites of these anti-Musashi1 Mabs are remarkably conserved among the human, mouse, and Xenopus proteins. Spatiotemporal patterns of Musashi1 immunoreactivity in the developing and/or adult CNS tissuesof frogs, birds, rodents, and humans indicated that our anti-Musashi1 Mabsreacted with undifferentiated, proliferative cells in nostaining of embryonic mouse brain cells in monolayer cultures demonstrated strong Musashi1 expression in Nestin(+)/RC2(+) cells. The relative number of Musashi1(+)/Nestin(+)/RC2(+) cells increased fivefold when embryonic forebrain cells were cultured to form 'neurospheres' in which stem-like cells are known to be enriched through their self-renewing mode of growth. Nestin(+)/RC2(-) cells, which included T alpha 1-GFP(+) neuronal progenitor cells and GLAST(+) astroglial precursor cells, were also Musashi1(+), as were GFAP(+) astrocytes. Young neurons showed a trace of Musashi1 expression. Cells committed to the oligodendroglial lineage were Musashi(-). Musashi1 was localized to the perikarya of CNS stem-like cells and non-oligodendroglial progenitor cells without shifting to cell processes or endfeet, and is therefore advantageous for identifying each cell and counting cells in situ. Copyright (C) 2000 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:45:24