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Titolo:
Caspase regulation of neuronal progenitor cell apoptosis
Autore:
DSa-Eipper, C; Roth, KA;
Indirizzi:
Washington Univ, Sch Med, Dept Pathol, Div Neuropathol, St Louis, MO 63110USA Washington Univ St Louis MO USA 63110 Neuropathol, St Louis, MO 63110USA
Titolo Testata:
DEVELOPMENTAL NEUROSCIENCE
fascicolo: 1-2, volume: 22, anno: 2000,
pagine: 116 - 124
SICI:
0378-5866(200001/04)22:1-2<116:CRONPC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPING NERVOUS-SYSTEM; X-DEFICIENT MICE; CYTOCHROME-C; FLOW-CYTOMETRY; DEATH; BAX; RELEASE; BCL-2; IDENTIFICATION; MITOCHONDRIAL;
Keywords:
caspase-3; caspase-9; Bax; programmed cell death; neuronal stem cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Roth, KA Washington Univ, Sch Med, Dept Pathol, Div Neuropathol, 600 S Euclid Ave,Box 8118, St Louis, MO 63110 USA Washington Univ 600 S Euclid Ave,Box 8118 St Louis MO USA 63110 A
Citazione:
C. D'Sa-Eipper e K.A. Roth, "Caspase regulation of neuronal progenitor cell apoptosis", DEV NEUROSC, 22(1-2), 2000, pp. 116-124

Abstract

Programmed cell death (apoptosis) of both proliferating neuroblasts and postmitotic neurons is essential for normal nervous system development. To study the molecular regulation of apoptosis in neuronal progenitor cells, we developed a flow cytometric assay capable of distinguishing between viable,apoptotic, and necrotic cell populations. Incubation of freshly dissociated telencephalic cells from gestational day 12-13 mouse embryos with either cytosine arabinoside (AraC) or staurosporine caused a marked increase in the percentage of apoptotic cells, Both drugs induced caspase-3 activation, as determined by in vitro cleavage of a caspase-3 substrate and immunocytochemical detection of activated caspase-3, Treatment of telencephalic cells with the broad caspase inhibitor BAF, blocked caspase-3 activation and protected cells against both AraC and staurosporine-induced apoptotic death. These results indicate that neuronal progenitors possess a caspase-dependent apoptotic pathway, the activation of which may regulate neuronal progenitor cell numbers in vivo. Copyright (C) 2000 S. Karger AG, Basel.

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Documento generato il 07/07/20 alle ore 22:14:00