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Titolo:
Serum immunoglobulin E levels predict human airway reactivity in vitro
Autore:
Schmidt, D; Watson, N; Ruehlmann, E; Magnussen, H; Rabe, KF;
Indirizzi:
Leiden Univ, Med Ctr, Dept Pulmonol, NL-2300 RA Leiden, Netherlands LeidenUniv Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands Krankenhaus Grosshansdorf, Zentrum Pneumol & Thoraxchirurg, LVA Hamburg, D-2070 Grosshansdorf, Germany Krankenhaus Grosshansdorf Grosshansdorf Germany D-2070 hansdorf, Germany
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 2, volume: 30, anno: 2000,
pagine: 233 - 241
SICI:
0954-7894(200002)30:2<233:SIELPH>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBSTRUCTIVE PULMONARY-DISEASE; SMOOTH-MUSCLE; BRONCHIAL RESPONSIVENESS; INVITRO RESPONSES; FLOW OBSTRUCTION; IGE LEVELS; PASSIVE SENSITIZATION; CIGARETTE SMOKERS; LUNG-FUNCTION; YOUNG-ADULTS;
Keywords:
asthma; bronchial hyperresponsiveness; COPD; human airway smooth muscle; immunoglobulin E; in vitro; in vivo; sensitization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Rabe, KF Leiden Univ, Med Ctr, Dept Pulmonol, C3-P,POB 9600, NL-2300 RA Leiden, Netherlands Leiden Univ C3-P,POB 9600 Leiden Netherlands NL-2300 RA herlands
Citazione:
D. Schmidt et al., "Serum immunoglobulin E levels predict human airway reactivity in vitro", CLIN EXP AL, 30(2), 2000, pp. 233-241

Abstract

Background Airway hyperresponsiveness to non-specific stimuli is one characteristic feature of airway diseases such as bronchial asthma and chronic bronchitis. Until now, studies aiming to demonstrate a relationship between in vivo conditions associated with airway hyperreactivity and in vitro airway responsiveness have been inconclusive. Objective Since serum immunoglobulin (Ig) E is believed to be one determinant of airway reactivity in vivo, we studied whether in vitro airway reactivity in lung resection material from patients with elevated levels of serumIgE was increased as compared with patients with undetectable IgE. By thisapproach, we aimed to elucidate the role of circulating IgE for bronchial smooth muscle reactivity in vitro. Methods Bronchial rings from nine patients with total serum IgE levels above 200 U/mL and 10 patients with total serum IgE levels below 10 U/mL were passively sensitized, i.e. incubated overnight with buffer or sensitizing serum containing high levels of total IgE (> 250 U/mL). Afterwards, contractile responses to histamine were assessed in the organ bath. Results Histamine responsiveness was significantly increased in airways obtained from patients with IgE levels above 200 U/mL as compared with airways from patients with IgE levels below 10 U/mL (P < 0.05). Passive sensitization of bronchi from patients with low IgE significantly increased histamine responsiveness, as compared with non-sensitized controls from the same patients (P < 0.05). In contrast, passive sensitization of airways from patients with elevated IgE did not further increase responsiveness. There was nodifference in histamine reactivity between non-passively sensitized and passively sensitized tissue preparations from patients with IgE above 200 U/mL and passively sensitized tissues from patients with IgE below 10 U/mL. Conclusion Our findings reveal that elevated levels of serum IgE predict airway hyperresponsiveness to histamine in vitro. At the same time, they indicate that the in vitro model of passive sensitization, in addition to its ability to induce allergen responses, also mimics conditions of non-specific airway hyperreactivity, which are relevant under in vivo conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/02/20 alle ore 11:17:32