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Titolo:
Quantitative detection of t(14;18)-positive cells in patients with follicular lymphoma before and after autologous bone marrow transplantation
Autore:
Hirt, C; Dolken, G;
Indirizzi:
Univ Greifswald, Klin & Poliklin Innere Med C, D-17487 Greifswald, GermanyUniv Greifswald Greifswald Germany D-17487 , D-17487 Greifswald, Germany
Titolo Testata:
BONE MARROW TRANSPLANTATION
fascicolo: 4, volume: 25, anno: 2000,
pagine: 419 - 426
SICI:
0268-3369(200002)25:4<419:QDOTCI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMA; POLYMERASE CHAIN-REACTION; BREAKPOINT-CLUSTER REGION; HIGH-DOSE THERAPY; CHROMOSOMAL BREAKPOINT; PROGNOSTIC FACTORS; B-CELLS; T(14-18); BLOOD; REARRANGEMENTS;
Keywords:
follicular lymphoma; autologous bone marrow transplantation; purging; t(14;18); MRD; real-time quantitative PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Dolken, G Univ Greifswald, Klin & Poliklin Innere Med C, Sauerbruchstr, D-17487 Greifswald, Germany Univ Greifswald Sauerbruchstr Greifswald GermanyD-17487 ermany
Citazione:
C. Hirt e G. Dolken, "Quantitative detection of t(14;18)-positive cells in patients with follicular lymphoma before and after autologous bone marrow transplantation", BONE MAR TR, 25(4), 2000, pp. 419-426

Abstract

The aim of this study was to evaluate whether a quantitative analysis of circulating t(14;18)-positive cells is of prognostic significance in patients with follicular lymphoma (FL) after myelo-ablative therapy supported by ABMT, We tested DNA from primary lymphoma tissue as well as PBMC before and after ABMT from 15 patients for the presence of the t(14;18) translocatican. Nine patients showed a t(14;18) translocation, six patients were t(14;18)-negative. Circulating t(14;18)positive cells of seven patients were quantitatively determined by limiting dilution assays combined with a two-step PCR and by real-time quantitative PCR, The results of both methods correlate very well. The number of circulating t(14;18)-positive cells decreased significantly in all patients after myeloablative therapy and ABMT, t(14;18)-negative blood samples were found in five of seven patients. In all patients circulating t(14;18)positive cells reappeared within 2 years after ABMT showing two different patterns. During continuous CR the numbers of circulating t(14;18)-positive cells were found to be stable within one order of magnitude. In contrast, in one patient the relapse was accompanied by a logarithmic increase of t(14;18)-positive cells. In a second patient the enlargement of lymph nodes developing over a period of 12 months was accompanied by very slowly increasing numbers of t(14;18)-positive cells. In all cases where diagnostic lymph node tissue was available, the same t(14;18) translocation was found at first diagnosis and after ABMT as shown by nucleotide sequence analysis. We conclude that the quantitative detection of circulating t(14;18)-positive cells during follow-up of patients with FL after ABMT reflects the clinical course of the disease. Relapses are associated with increasing numbers of circulating t(14;18)-positive cells and continuous completeremissions with stable cell counts.

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Documento generato il 01/12/20 alle ore 07:42:28