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Titolo:
Characterization of CD34(+) subsets derived from bone marrow, umbilical cord blood and mobilized peripheral blood after stem cell factor and interleukin 3 stimulation
Autore:
De Bruyn, C; Delforge, A; Lagneaux, L; Bron, D;
Indirizzi:
Inst Jules Bordet, Serv Med Interne, B-1000 Brussels, Belgium Inst Jules Bordet Brussels Belgium B-1000 erne, B-1000 Brussels, Belgium Inst Jules Bordet, Lab Rech & Invest Clin, B-1000 Brussels, Belgium Inst Jules Bordet Brussels Belgium B-1000 Clin, B-1000 Brussels, Belgium
Titolo Testata:
BONE MARROW TRANSPLANTATION
fascicolo: 4, volume: 25, anno: 2000,
pagine: 377 - 383
SICI:
0268-3369(200002)25:4<377:COCSDF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
EX-VIVO EXPANSION; HUMAN HEMATOPOIETIC PROGENITORS; CULTURE-INITIATING CELLS; C-KIT RECEPTOR; FUNCTIONAL DIFFERENCES; CYCLING STATUS; IN-VIVO; HLA-DR; EXPRESSION; SUBPOPULATIONS;
Keywords:
c-kit; cell cycle; cord blood; bone marrow; peripheral blood;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Bron, D Inst Jules Bordet, Serv Med Interne, Rue Heger Bordet 1, B-1000 Brussels, Belgium Inst Jules Bordet Rue Heger Bordet 1 Brussels Belgium B-1000 gium
Citazione:
C. De Bruyn et al., "Characterization of CD34(+) subsets derived from bone marrow, umbilical cord blood and mobilized peripheral blood after stem cell factor and interleukin 3 stimulation", BONE MAR TR, 25(4), 2000, pp. 377-383

Abstract

We characterized CD34(+) cells purified from bone marrow (BM), mobilized peripheral blood (PB) and cord blood (CB) and we tried to establish correlations between the cell cycle kinetics of the CD34(+)CD38(-) and CD34(+)CD38() subpopulations, their sensitivity to SCF and IL-3 and their expression of receptors for these two CSFs. At day 0, significantly fewer immature CD34(+)CD38(-) cells from CB and mobilized PB are in S + G2M phases of the cellcycle (respectively 2.0 +/- 0.4 and 0.9 +/- 0.3 %) than their BM counterpart (5.6 +/- 1.2%). A 48-h incubation with SCF + IL-3 allows a significant increase in the percentage of carding CD34(+)CD38(-) cells in CB (19.2 +/- 2.2%, P < 0.0002) and PB (14.1 +/- 5.5%, P<0.05) while the proliferative potential of BM CD34(+)CD38(-) progenitors remains constant (8.6 +/- 1.0%, NS). CD123 (IL-3 receptor) expression is similar in the three sources of hematopoietic cells at day 0 and after 48-h culture. CD117 (SCF receptor) expression, although very heterogeneous according to the subpopulations and the sources of progenitors evaluated, seems not to correlate with the differenceof progenitor cell sensitivity to SCF nor with their proliferative capacity. Considering the importance of the c-kit/SCF complex in the adhesion of stem cells to the microenvironment, several observations are relevant. The density of CD117 antigen expression (expressed in terms of mean equivalent soluble fluorescence, MESF) is significantly lower on fresh PB cells than ontheir BM (P < 0.017) and CB (P < 0.004) counterparts, particularly in the immature CD34(+)CD38(-) population (560 +/- 131, 2121 +/- 416 and 1192 +/-129 MESF respectively); moreover, when PB and BM CD34(+)CD38(-) cells are stimulated for 48 h with SCF + IL-3, the CD117 expression decreases by 1.5- and 1.66-fold, respectively. This reduction could modify the functional capacities of ex vivo PB and BM manipulated immature progenitor cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 10:21:58