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Titolo:
Assay and importance of adhesive interaction between hamster (Mesocricetusauratus) oocyte-cumulus complexes and the oviductal epithelium
Autore:
Lam, X; Gieseke, C; Knoll, M; Talbot, P;
Indirizzi:
Univ Calif Riverside, Dept Neurosci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 osci, Riverside, CA 92521 USA
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 3, volume: 62, anno: 2000,
pagine: 579 - 588
SICI:
0006-3363(200003)62:3<579:AAIOAI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACROSOME REACTION; IN-VITRO; RABBIT OVIDUCT; OVUM PICKUP; OOPHORUS; SPERM; SPERMATOZOA; BOVINE; CELLS; CILIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Talbot, P Univ Calif Riverside, Dept Neurosci, Riverside, CA 92521 USA Univ Calif Riverside Riverside CA USA 92521 rside, CA 92521 USA
Citazione:
X. Lam et al., "Assay and importance of adhesive interaction between hamster (Mesocricetusauratus) oocyte-cumulus complexes and the oviductal epithelium", BIOL REPROD, 62(3), 2000, pp. 579-588

Abstract

Adhesion between the oocyte-cumulus complex and infundibulum plays an important, but poorly understood, role in oocyte pick-up. The purposes of this study were to determine which components of thtr oocyte-cumulus complex andoviductal epithelium function in adhesion, to measure adhesion under physiological conditions, and to examine the effect of modulation of adhesion onoocyte-cumulus complex pick-up rate. Oocyte-cumulus complexes containing an expanded matrix were readily transported into the oviduct, while unexpended complexes lacking an extracellular matrix were not picked up, indicatingthat the matrix is necessary for pick-up. Transmission electron microscopyrevealed that during pick-up, adhesion occurred specifically between the ciliary crowns of the oviduct and the granules and filaments of the cumulus matrix. An assay was developed using vacuum from a low-flow peristaltic precision pump, modified for hi-directional flow, to measure the strength of adhesion between the oocyte-cumulus complex and the oviductal epithelium, and adhesion was measured during physiological conditions. The lectin wheat germ agglutinin and the polycation poly-L-lysine were then used to modulate adhesion, and the effects of increasing or decreasing adhesion on oocyte pick-up rate and ciliary beat frequency were examined, The data show that 1) the matrix of the oocyte-cumulus complex and the ciliary crowns of the oviduct function in adhesion during pick-up and that adhesion is necessary for pick-up, 2) adhesion can be assayed quantitatively and is very uniform among control infundibula, and 3) decreasing or increasing adhesion decreases oocyte pick-up rate and in some cases prevents pickup without affecting ciliary beat frequency.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:43:26