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Titolo:
APPLICATION OF P450RGS (REPORTER GENE SYSTEM) AS A BIOINDICATOR OF SEDIMENT PAH CONTAMINATION IN THE VICINITY OF INCHEON HARBOR, KOREA
Autore:
KIM GB; ANDERSON JW; BOTHNER K; LEE JH; KOH CH; TANABE S;
Indirizzi:
EHIME UNIV,DEPT ENVIRONM CONSERVAT,TARUMI 3-5-7 MATSUYAMA EHIME 790 JAPAN COLUMBIA ANALYT SERV CARLSBAD CA 92009 MEC ANALYT SYST CARLSBAD CA 92009 SEOUL NATL UNIV,DEPT OCEANOG SEOUL 151742 SOUTH KOREA
Titolo Testata:
Biomarkers
fascicolo: 3, volume: 2, anno: 1997,
pagine: 181 - 188
SICI:
1354-750X(1997)2:3<181:AOP(GS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCYCLIC AROMATIC-HYDROCARBONS; FISH; CYTOCHROME-P-450; EXPOSURE; SOILS; BIOMARKER; INDUCTION; TRANSPORT; FLATFISH; ATLANTIC;
Keywords:
CYP1A1; P450RGS BAPTEQ; PAH; BIOINDICATOR; SCREENING METHOD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
G.B. Kim et al., "APPLICATION OF P450RGS (REPORTER GENE SYSTEM) AS A BIOINDICATOR OF SEDIMENT PAH CONTAMINATION IN THE VICINITY OF INCHEON HARBOR, KOREA", Biomarkers, 2(3), 1997, pp. 181-188

Abstract

Sixty-seven sediment samples were collected from Kyeonggi Bay, Korea,including the mouth of Han River Incheon Harbor, the Namdong industrial complex, and the open sea. Collections were conducted in December, 1995 and samples were maintained frozen (-20 degrees C) until analysis. Dichloromethane extracts were analysed for their content of CYP1A1-inducing compounds with a P450RGS (reporter gene system) assay, and forpolycyclic aromatic hydrocarbons (PAHs). Sediment samples were also analysed for organic carbon (OC) content and grain size, to aid in evaluating the relationship between contamination and physical nature of the sediments. The responses of the P450RGS assay to the sediment extracts were expressed as mu g of benzo[a]pyrene toxic equivalents per g dry weight (mu g g(-1) BaPTEQ), and these values correlated well (r(2)=0.624) with total PAHs. BaPTEQ values were also highly correlated withthe OC content of the sediments. The determination of P450RGS BaPTEQ is a useful tool, because it is both a rapid and inexpensive means of assessing the potential toxicity of organic compounds in environmentalsediment samples. These values represent an estimate of the levels ofcompounds in the sediment that are potentially available to organismsthrough chronic exposure to pore water or ingestion of benthic species, We believe BaPTEQ values are more useful than tables of specific PAH concentrations, if the purpose of the investigation is to either obtain a rapid screening of an area or to develop some form of ecologicalor human health risk assessment.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 11:41:25