Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Genetic organization of the mobilization region of the plasmid pHE1 from Halomonas elongata
Autore:
Vargas, C; Tegos, G; Vartholomatos, G; Drainas, C; Ventosa, A; Nieto, JJ;
Indirizzi:
Univ Seville, Fac Pharm, Dept Microbiol & Parasitol, E-41012 Seville, Spain Univ Seville Seville Spain E-41012 l & Parasitol, E-41012 Seville, Spain Univ Ioannina, Dept Chem, Sector Organ Chem & Biochem, GR-45110 Ioannina, Greece Univ Ioannina Ioannina Greece GR-45110 iochem, GR-45110 Ioannina, Greece
Titolo Testata:
SYSTEMATIC AND APPLIED MICROBIOLOGY
fascicolo: 4, volume: 22, anno: 1999,
pagine: 520 - 529
SICI:
0723-2020(199912)22:4<520:GOOTMR>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MODERATELY HALOPHILIC EUBACTERIA; ESCHERICHIA-COLI; SHUTTLE VECTOR; HOST-RANGE; DNA; PROTEIN; BACTERIA; ORIGIN; SEQUENCE; CONSTRUCTION;
Keywords:
moderate halophiles; Halomonas elongata; plasmid mobilization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Nieto, JJ Univ Seville, Fac Pharm, Dept Microbiol & Parasitol, E-41012 Seville, Spain Univ Seville Seville Spain E-41012 tol, E-41012 Seville, Spain
Citazione:
C. Vargas et al., "Genetic organization of the mobilization region of the plasmid pHE1 from Halomonas elongata", SYST APPL M, 22(4), 1999, pp. 520-529

Abstract

The mobilization (mob) region of the non-self transmissible 4.2-kb plasmidpHE1 from the moderately halophilic bacterium Halomonas elongata ATCC 33174 has been identified and characterized. Analysis of the sequence revealed the presence of four open reading frames (mobCABD) which show a complex organization with two of them (mobB and mobD) entirely overlapped by a third (mobA). The deduced proteins appeared to have a high degree of homology to Mob proteins of CoIE1 and closely related plasmids. To assess the functionality of the mob region, the hybrid vector pHS134 was constructed, consistingof the complete plasmid pHE1, the E. coil vector pKS(-) and a streptomycin-resistance gene for positive selection in Halomonas. Vector pHS134 was found to bs mobilizable from E. soli to It elongata assisted by pRK600. Upstream of the mob genes, an oriT region with a putative nick sequence highly homologous to that of CoIE1 plasmids was identified. To our knowledge, this is the first mobilizable plasmid found in moderate halophiles. This property, together with its small size, the availability of its complete sequence, and its broad host range: in moderately halophilic strains, makes pHF1 a good candidate for the construction of cloning and expression vectors for these extremophiles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 02:31:06