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Titolo:
Two novel Drosophila TAF(II)s have homology with human TAF(II)30 and are differentially regulated during development
Autore:
Georgieva, S; Kirschner, DB; Jagla, T; Nabirochkina, E; Hanke, S; Schenkel, H; De Lorenzo, C; Sinha, P; Jagla, K; Mechler, B; Tora, L;
Indirizzi:
ULP, CNRS, INSERM, Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch, CU Strasbourg, France ULP Illkirch CU Strasbourg France F-67404 Illkirch, CU Strasbourg, France INSERM, U384, F-63001 Clermont Ferrand, France INSERM Clermont Ferrand France F-63001 F-63001 Clermont Ferrand, France Russian Acad Sci, Inst Gene Biol, Moscow, Russia Russian Acad Sci MoscowRussia Acad Sci, Inst Gene Biol, Moscow, Russia Univ Oslo, Ctr Med Studies, Moscow, Russia Univ Oslo Moscow RussiaUniv Oslo, Ctr Med Studies, Moscow, Russia Deutsch Krebsforschungszentrum, Dept Dev Genet, D-69120 Heidelberg, Germany Deutsch Krebsforschungszentrum Heidelberg Germany D-69120 lberg, Germany Drosophila Stock Ctr, Sch Life Sci, Indore 452001, Madhya Pradesh, India Drosophila Stock Ctr Indore Madhya Pradesh India 452001 ya Pradesh, India
Titolo Testata:
MOLECULAR AND CELLULAR BIOLOGY
fascicolo: 5, volume: 20, anno: 2000,
pagine: 1639 - 1648
SICI:
0270-7306(200003)20:5<1639:TNDTHH>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; TRANSCRIPTIONAL ACTIVATORS; MOLECULAR-CLONING; ACETYLASE COMPLEX; BINDING PROTEIN; TFIID SUBUNIT; IN-VIVO; GENE; DISTINCT; TAFS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Tora, L ULP, CNRS, INSERM, Inst Genet & Biol Mol & Cellulaire, BP 163, F-67404 Illkirch, CU Strasbourg, France ULP BP 163 Illkirch CU Strasbourg France F-67404 trasbourg, France
Citazione:
S. Georgieva et al., "Two novel Drosophila TAF(II)s have homology with human TAF(II)30 and are differentially regulated during development", MOL CELL B, 20(5), 2000, pp. 1639-1648

Abstract

TFIID is a multiprotein complex composed of the TATA binding protein (TBP)and TBP-associated factors (TAF(II)s). The binding of TFIID to the promoter is the first step of RNA polymerase II preinitiation complex assembly on protein-coding genes. Yeast Oil and human (h) TFIID complexes contain 10 to13 TAF(II)s. Biochemical studies suggested that the Drosophila (d) TFIID complexes contain only eight TAF(II)s, leaving a number of yeast and human TAF(II)s (e.g., hTAF(II)55, hTAF(II)30, and hTAF(II)18) without known Drosophila homologues. We demonstrate that Drosophila has not one but two hTAF(II)30 homologues, dTAF(II)16 and dTAF(II)24, which are encoded by two adjacent genes. These two genes are localized in a head-to-head orientation, and their 5' extremities overlap. We show that these novel dTAF(II)s are expressed and that they are both associated with TBP and other bona fide dTAF(II)sin dTF(II)D complexes. dTAF(II)24, but not dTAF(II)16, was also found to be associated with the histone acetyltransferase (HAT) dGCN5. Thus, dTAF(II)16 and dTAF(II)24 are functional homologues of hTAF(II)30, and this is the first demonstration that a TAF(II)-GCN5-HAT complex exists in Drosophila. The two dTAF(II)s are differentially expressed during embryogenesis and can be detected in both nuclei and cytoplasm of the cells. These results together indicate that dTAF(II)16 and dTAF(II)24 may have similar but not identical functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/21 alle ore 04:11:44