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Titolo:
2-(4-carboxyphenyl)-6-N,N-diethylaminobenzofuran: a useful reagent for thesensitive determination of alcohols by high-performance liquid chromatography with fluorimetric detection
Autore:
Assaf, P; Katzhendler, J; Haj-Yehia, AI;
Indirizzi:
Hebrew Univ Jerusalem, Sch Pharm, David R Bloom Ctr Pharm, Dept Pharmaceut, IL-91120 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91120 -91120 Jerusalem, Israel Hebrew Univ Jerusalem, Sch Pharm, David R Bloom Ctr Pharm, Dept Med Chem, IL-91120 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91120 -91120 Jerusalem, Israel
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY A
fascicolo: 1-2, volume: 869, anno: 2000,
pagine: 243 - 250
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE DERIVATIZATION REAGENT; PRECOLUMN DERIVATIZATION; QUANTITATIVE-ANALYSIS; ACIDS;
Keywords:
derivatization, LC; alcohols; carboxyphenyldiethylaminobenzofuran;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Haj-Yehia, AI Hebrew Univ Jerusalem, Sch Pharm, David R Bloom Ctr Pharm, Dept Pharmaceut, POB 12065, IL-91120 Jerusalem, Israel Hebrew Univ JerusalemPOB 12065 Jerusalem Israel IL-91120 l
Citazione:
P. Assaf et al., "2-(4-carboxyphenyl)-6-N,N-diethylaminobenzofuran: a useful reagent for thesensitive determination of alcohols by high-performance liquid chromatography with fluorimetric detection", J CHROMAT A, 869(1-2), 2000, pp. 243-250

Abstract

A simple and highly sensitive method for the determination of short, medium and long-chain alcohols using highperformance liquid chromatography with fluorimetric detection is described. The alcohols were derivatized to theircorresponding esters with (4-carboxyphenyl)-6-N,N-diethylaminobenzofuran. The esterification reaction proceeded rapidly and smoothly in acetonitrile at 60 degrees C with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (a coupling agent) in the presence of 4-dimethylaminopyridine (a base catalyst). The resulting esters of alcohols from methanol to eicosanol (C-1-C-20-ol) were separated on a reversed-phase column (Ultrasphere C-8) with gradient elution (acetonitrile-water) and detected fluorometrically (excitation 387, emission 537 nm). The lower limits of detection (signal-to-noise ratio of 3) for the derivatized alcohols were in the range of 0.2-0.5 pg. (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:36:19