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Titolo:
Constitutive and inducible nitric oxide synthases after dynorphin-induced spinal cord injury
Autore:
Hu, WH; Qiang, WA; Li, F; Liu, N; Wang, GQ; Wang, HY; Wan, XCST; Liao, WH; Liu, JS; Jen, MF;
Indirizzi:
Third Mil Med Univ, Inst Surg Res, Dept Spinal Cord Injury, Chongqing 400042, Peoples R China Third Mil Med Univ Chongqing Peoples R China 400042 042, Peoples R China Third Mil Med Univ, Daping Hosp, Chongqing 400042, Peoples R China Third Mil Med Univ Chongqing Peoples R China 400042 042, Peoples R China Peking Union Med Coll, Inst Basic Med Sci, Dept Pharmacol, Beijing 100005,Peoples R China Peking Union Med Coll Beijing Peoples R China 100005 005,Peoples R China Peking Union Med Coll, Inst Basic Med Sci, Dept Neurobiol, Beijing 100005,Peoples R China Peking Union Med Coll Beijing Peoples R China 100005 005,Peoples R China Chinese Acad Med Sci, Beijing 100005, Peoples R China Chinese Acad Med Sci Beijing Peoples R China 100005 005, Peoples R China
Titolo Testata:
JOURNAL OF CHEMICAL NEUROANATOMY
fascicolo: 4, volume: 17, anno: 2000,
pagine: 183 - 197
SICI:
0891-0618(200001)17:4<183:CAINOS>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOCAL CEREBRAL-ISCHEMIA; TRAUMATIC BRAIN INJURY; CENTRAL-NERVOUS-SYSTEM; DORSAL HORN NEURONS; NADPH-DIAPHORASE; MESSENGER-RNA; OPIOID RECEPTORS; NEONATAL RAT; GLIAL-CELLS; EXPRESSION;
Keywords:
nitric oxide; nitric oxide synthase; dynorphin; spinal cord injury; neurotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
84
Recensione:
Indirizzi per estratti:
Indirizzo: Hu, WH Third Mil Med Univ, Inst Surg Res, Dept Spinal Cord Injury, Chongqing 400042, Peoples R China Third Mil Med Univ Chongqing Peoples R China 400042 oples R China
Citazione:
W.H. Hu et al., "Constitutive and inducible nitric oxide synthases after dynorphin-induced spinal cord injury", J CHEM NEUR, 17(4), 2000, pp. 183-197

Abstract

It has recently been demonstrated that selective inhibition of both neuronal constitutive and inducible nitric oxide synthases (ncNOS and iNOS) is neuroprotective in a model of dynorphin (Dyn) A(1-17)-induced spinal cord injury. In the present study, various methods including the conversion of H-3-L-arginine to H-3-citrulline, immunohistochemistry and in situ hybridization are employed to determine the temporal profiles of the enzymatic activities, immunoreactivities, and mRNA expression for both ncNOS and iNOS after intrathecal injection of a neurotoxic dose (20 nmol) of Dyn A(1-17). The expression of ncNOS immunoreactivity and mRNA increased as early as 30 min after injection and persisted for 1-4 h. At 24-48 h, the number of ncNOS positive cells remained elevated while most neurons died. The cNOS enzymatic activity in the ventral spinal cord also significantly increased at 30 min-48 h, but no significant changes in the dorsal spinal cord were observed. However, iNOS mRNA expression increased later at 2 h, iNOS immunoreactivity andenzymatic activity increased later at 4 h and persisted for 24-48 h after injection of 20 nmol Dyn A(1-17). These results indicate that both ncNOS and iNOS are associated with Dyn-induced spinal cord injury, with ncNOS predominantly involved at an early stags and iNOS at a later stage: (C) 2000 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 22:04:41