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Titolo:
Development of an in situ mouse brain perfusion model and its application to mdr1a P-glycoprotein-deficient mice
Autore:
Dagenais, C; Rousselle, C; Pollack, GM; Scherrmann, JM;
Indirizzi:
Hop Fernand Widal, INSERM, U26, F-75475 Paris 10, France Hop Fernand Widal Paris France 10 INSERM, U26, F-75475 Paris 10, France Univ N Carolina, Sch Pharm, Div Drug Delivery & Disposit, Chapel Hill, NC USA Univ N Carolina Chapel Hill NC USA ivery & Disposit, Chapel Hill, NC USA
Titolo Testata:
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
fascicolo: 2, volume: 20, anno: 2000,
pagine: 381 - 386
SICI:
0271-678X(200002)20:2<381:DOAISM>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-PROTEIN BINDING; OVARY CELL MUTANTS; BLOOD; BARRIER; RAT; TRANSPORT; PERMEABILITY; COLCHICINE; GLUCOSE; PHARMACOKINETICS;
Keywords:
blood-brain barrier; P-glycoprotein; brain uptake; transporters;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Scherrmann, JM Hop Fernand Widal, INSERM, U26, 200 Rue Faubourg St Denis, F-75475 Paris 10, France Hop Fernand Widal 200 Rue Faubourg St Denis ParisFrance 10
Citazione:
C. Dagenais et al., "Development of an in situ mouse brain perfusion model and its application to mdr1a P-glycoprotein-deficient mice", J CEREBR B, 20(2), 2000, pp. 381-386

Abstract

An in situ mouse brain perfusion model predictive of passive and carrier-mediated transport across the blood-brain barrier (BBB) was developed and applied to mdr1a P-glycoprotein (Pgp)-deficient mice [mdr1a(-/-)]. Cerebral flow was estimated from diazepam uptake. Physical integrity of the BBB was assessed with sucrose/inulin spaces; functional integrity was assessed with glucose uptake, which was: saturable with a K-m of similar to 17 mmol/L andV-max of 310 mmol . 100 g(-1). min(-1). Brain uptake of a Pgp substrate (colchicine) was significantly enhanced (two- to fourfold) in mdr1a(-/-) mice. These data suggest that the model is applicable to elucidating the effects of efflux transporters, including Pgp, on brain uptake.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:22:59