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Titolo:
The suitability of [C-11]-alpha-methyl-L-tryptophan as a tracer for serotonin synthesis: Studies with dual administration of [C-11] and [C-14] labeled tracer
Autore:
Shoaf, SE; Carson, RE; Hommer, D; Williams, WA; Higley, JD; Schmall, B; Herscovitch, P; Eckelman, WC; Linnoila, M;
Indirizzi:
NIAAA, DICBR, Clin Studies Lab, Bethesda, MD 20892 USA NIAAA Bethesda MD USA 20892 CBR, Clin Studies Lab, Bethesda, MD 20892 USA NIH, PET Dept, Ctr Clin, Bethesda, MD 20892 USA NIH Bethesda MD USA 20892NIH, PET Dept, Ctr Clin, Bethesda, MD 20892 USA
Titolo Testata:
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
fascicolo: 2, volume: 20, anno: 2000,
pagine: 244 - 252
SICI:
0271-678X(200002)20:2<244:TSO[AA>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON-EMISSION-TOMOGRAPHY; METHYL-L-TRYPTOPHAN; HUMAN BRAIN; SYNTHESIS INVIVO; SYNTHESIS RATES; RHESUS-MONKEYS; IN-VIVO; ALPHA-METHYL-L-TRYPTOPHAN; KINETICS; PET;
Keywords:
serotonin; positron emission tomography; rhesus monkeys; pharmacokinetics; brain; alpha-methyl-L-tryptophan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Shoaf, SE OAPI, 2440 Res Blvd, Rockville, MD 20850 USA OAPI 2440 Res Blvd Rockville MD USA 20850 ockville, MD 20850 USA
Citazione:
S.E. Shoaf et al., "The suitability of [C-11]-alpha-methyl-L-tryptophan as a tracer for serotonin synthesis: Studies with dual administration of [C-11] and [C-14] labeled tracer", J CEREBR B, 20(2), 2000, pp. 244-252

Abstract

The tracer [C-11]-alpha-methyl-L-tryptophan (alpha MTP) has been used to measure brain serotonin synthesis rates with positron emission tomography (PET). To address questions about the accuracy of the kinetic model, [C-14]alpha MTP was used to directly measure conversion to [C-14]-alpha-methyl-serotonin (alpha M5HT) in monkeys that had been previously studied with PET and[C-11]alpha MTP. Four male, fasted, isonurane-anesthetized rhesus monkeys were studied with [C-11]aMTP and PET. Immediately after the initial 3-hour scan, a second dose of [C-11]alpha MTP was coinjected with 1 mCi of [C-14]alpha MTP, and additional PET data were collected. Approximately 90 minutes after the second aMTP administration. the animals were killed with an overdose of phenobarbital. and brain samples from 21 regions were taken and analyzed by HPLC. Minimal conversion of alpha MTP to alpha M5HT occurred; HPLC analysis of C-14 radioactivity showed that greater than 96% of the total counts were in fractions corresponding to the alpha MTP peak. Brain concentrations of serotonin, tryptophan, 5-hydroxyindole-3-acetic acid, and alpha MTP also were determined fluorometrically using exernal quantification. Patlak plots generated from PET images acquired over 3 hours showed no time period of linear increase, and final slopes were not significantly different from zero, consistent With the finding of minimal conversion to [C-14]alpha M5HT. These data indicate that in the 3-hour period after injection, [C-11]alpha MTP is acting predominantly as a tracer of tryptophan uptake, not serotonin synthesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 08:41:11