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Titolo:
Characterization and role of tbuX in utilization of toluene by Ralstonia pickettii PKO1
Autore:
Kahng, HY; Byrne, AM; Olsen, RH; Kukor, JJ;
Indirizzi:
Rutgers State Univ, Biotechnol Ctr Agr & Environm, New Brunswick, NJ 08901USA Rutgers State Univ New Brunswick NJ USA 08901 New Brunswick, NJ 08901USA Rutgers State Univ, Dept Environm Sci, New Brunswick, NJ 08901 USA RutgersState Univ New Brunswick NJ USA 08901 New Brunswick, NJ 08901 USA Univ Michigan, Sch Med, Dept Microbiol & Immunol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 l & Immunol, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF BACTERIOLOGY
fascicolo: 5, volume: 182, anno: 2000,
pagine: 1232 - 1242
SICI:
0021-9193(200003)182:5<1232:CAROTI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PSEUDOMONAS TOL PLASMID; ENHANCER-BINDING PROTEINS; FATTY-ACID TRANSPORT; UPPER PATHWAY OPERON; NUCLEOTIDE-SEQUENCE; MEMBRANE-PROTEIN; TRANSCRIPTIONAL ACTIVATORS; HYPOXIC ENVIRONMENTS; FUNCTIONAL-ANALYSIS; PHENOL HYDROXYLASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Kukor, JJ Rutgers State Univ, Biotechnol Ctr Agr & Environm, Foran Hall,Cook Coll Campus,59 Dudley Rd, New Brunswick, NJ 08901 USA Rutgers State UnivForan Hall,Cook Coll Campus,59 Dudley Rd New Brunswick NJ USA 08901
Citazione:
H.Y. Kahng et al., "Characterization and role of tbuX in utilization of toluene by Ralstonia pickettii PKO1", J BACT, 182(5), 2000, pp. 1232-1242

Abstract

The tbu regulon of Ralstonia pickettii PKO1 encodes enzymes involved in the catabolism of toluene, benzene, and related alkylaromatic hydrocarbons, The first operon in this regulon contains genes that encode the tbu pathway's initial catabolic enzyme, toluene-3-monooxygenase, as well as TbuT, the NtrC-like transcriptional activator for the entire regulon. It has been previously shown that the organization of tbuT, which is located immediately downstream of tbuA1UBVA2C, and the associated promoter (PtbuA1) is unique in that it results in a cascade type of up-regulation of tbuT in response to avariety of effector compounds. In our efforts to further characterize thisunusual mode of gene regulation, we discovered another open reading frame,encoded on the strand opposite that of tbuT, 63 bp downstream of the tbu Tstop codon, The 1,374-bp open reading frame, encoding a 458-amino-acid peptide, was designated tbuX: The predicted amino acid sequence of TbuX exhibited significant similarity to several putative outer membrane proteins fromaromatic hydrocarbon-degrading bacteria, as well as to FadL, an outer membrane protein needed for uptake of long-chain fatty acids in Escherichia coli. Based on sequence analysis, transcriptional and expression studies, and deletion analysis, TbuX seems to play an important role in the catabolism of toluene in X. pickettii PKO1. In addition, the expression of tbuX appearsto be regulated in a manner such that low levels of TbuX are always present within the cell, whereas upon toluene exposure these levels dramatically increase, even more than those of toluene-3-monooxygenase. This expression pattern may relate to the possible role of TbuX as a facilitator of tolueneentry into the cell.

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Documento generato il 15/01/21 alle ore 22:30:41