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Titolo:
Chemokine- and cytokine-induced expression of endothelin 1 and endothelin-converting enzyme 1 in endothelial cells
Autore:
Molet, S; Furukawa, K; Maghazechi, A; Hamid, Q; Giaid, A;
Indirizzi:
Montreal Gen Hosp, Montreal, PQ H3G 1A4, Canada Montreal Gen Hosp Montreal PQ Canada H3G 1A4 Montreal, PQ H3G 1A4, Canada McGill Univ, Meakins Christie Labs, Montreal, PQ, Canada McGill Univ Montreal PQ Canada akins Christie Labs, Montreal, PQ, Canada Univ Oslo, Dept Anat, N-0316 Oslo, Norway Univ Oslo Oslo Norway N-0316Univ Oslo, Dept Anat, N-0316 Oslo, Norway
Titolo Testata:
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
fascicolo: 2, volume: 105, anno: 2000,
parte:, 1
pagine: 333 - 338
SICI:
0091-6749(200002)105:2<333:CACEOE>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOATTRACTANT; BIG ENDOTHELIN-1; TNF-ALPHA; FAMILY; ACTIVATION; PROTEIN-1; INDUCTION; RELEASE; RANTES;
Keywords:
endothelin; endothelin-converting enzyme 1; endothelial cells; Northern blot; messenger RNA; chemokines; cytokines; radioimmunoassay; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Giaid, A Montreal Gen Hosp, Suite L3-314,1650 Cedar Ave, Montreal, PQ H3G 1A4, Canada Montreal Gen Hosp Suite L3-314,1650 Cedar Ave Montreal PQ Canada H3G 1A4
Citazione:
S. Molet et al., "Chemokine- and cytokine-induced expression of endothelin 1 and endothelin-converting enzyme 1 in endothelial cells", J ALLERG CL, 105(2), 2000, pp. 333-338

Abstract

Background: Endothelin 1 (ET-1) is a product of endothelial and many othercell types that possesses a wide range of actions, including vasoconstriction, bronchoconstriction, and mitogenic activity on smooth muscle cells andfibroblasts. ET-1 release and expression is induced in several disease conditions associated with inflammation and cellular injury. Objective: The purpose of this study is to determine the effects of alpha-chemokines (IL-8 and melanoma growth-stimulating activator), beta-chemokines (monocyte chemotactic protein 1, macrophage inflammatory protein 1 alpha [MIP-1 alpha], MIP-1 beta, and RANTES), and proinflammatory cytokines (IL-1beta, TNF-alpha, and IFN-gamma) on the expression of both ET-1 and endothelin-converting enzyme 1 (ECE-1) by human umbilical vein endothelial cells. Methods: Subconfluent monolayers of human umbilical vein endothelial cellswere incubated with each chemokine individually for 24 hours or with a mixture (cytomix) of TNP-alpha, IL-1 beta, and IFN-gamma for 6 and 24 hours. Results: Incubation with the alpha-chemokines melanoma growth-stimulating activity and IL-8 did not significantly increase ET-1 and ECE-1 messenger (m)RNA expression and had no effect on ET-1 release. Monocyte chemotactic protein 1 exerted the most potent increase in ET-1 and ECE-1 mRNA and ET-1 release among all chemokines studied (P < .05), MTP-1 alpha and RANTES exerted a moderate, but significant, increase on the ET system (P < .05). The cytomix resulted in a significant increase in ET-1 and ECE-1 mRNA expression (P < .05). Conclusion: These data demonstrate that, like cytokines, chemokines can induce endothelial ET-1 and ECE-1 in vitro and suggest a possible role for these inflammatory mediators in the induction of the ET system in inflammatory and vascular diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 02:33:38