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Titolo:
Mycobacterium tuberculosis lipid antigens: use of multi-antigen based enzyme immunoassay for free and complex dissociated antibodies
Autore:
Harrington, JJ; Ho, JL; Silva, JRLE; Conde, MB; Kritski, AL; Fonseca, LS; Saad, MHF;
Indirizzi:
Fundacao Oswaldo Cruz, Setor Hanseniase, Lab Hanseniase, BR-21045900 Rio De Janeiro, Brazil Fundacao Oswaldo Cruz Rio De Janeiro Brazil BR-21045900BCaneiro, Brazil Cornell Univ, Coll Med, Ithaca, NY 14853 USA Cornell Univ Ithaca NY USA 14853 ell Univ, Coll Med, Ithaca, NY 14853 USA Hosp Univ Clementino Fraga Filho, Rio De Janeiro, Brazil Hosp Univ Clementino Fraga Filho Rio De Janeiro Brazil Janeiro, Brazil Univ Fed Rio de Janeiro, Inst Microbiol, BR-21941 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941 e Janeiro, Brazil
Titolo Testata:
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
fascicolo: 2, volume: 4, anno: 2000,
pagine: 161 - 167
SICI:
1027-3719(200002)4:2<161:MTLAUO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CIRCULATING IMMUNE-COMPLEXES; SL-IV; SEROLOGICAL DIAGNOSIS; SERONEGATIVE PATIENTS; HUMORAL RESPONSE; SERODIAGNOSIS; GLYCOLIPIDS; LEPROSY; SERA; DAT;
Keywords:
tuberculosis; lipid antigens; immunocomplexes; immunoassay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Saad, MHF Fundacao Oswaldo Cruz, Setor Hanseniase, Lab Hanseniase, Av Brasil 4365, BR-21045900 Rio De Janeiro, Brazil Fundacao Oswaldo Cruz Av Brasil4365 Rio De Janeiro Brazil BR-21045900 BC
Citazione:
J.J. Harrington et al., "Mycobacterium tuberculosis lipid antigens: use of multi-antigen based enzyme immunoassay for free and complex dissociated antibodies", INT J TUBE, 4(2), 2000, pp. 161-167

Abstract

OBJECTIVE: TO test the sensitivity and specificity of four lipid antigens of Mycobacterium tuberculosis: BDA-TBA, DAT, SL-I, and PIMs, adsorbed in the same microplate well, to detect reactive IgG by enzyme-immunoassay (EIA) from plain serum (MA-EIA) and dissociated immune complexes (ICMA-EIA). DESIGN: IgG antibodies against four antigens, placed in the same microplate well, were evaluated in serum from 155 tuberculous (TB) cases non-infected with the human immunodeficiency virus (HIV): 78 patients with positive bacilloscopy and culture, 33 patients with positive culture and 44 patients diagnosed by clinical and radiological criteria; and from 211 HIV negative control subjects: 32 patients with other pulmonary diseases, 100 healthy people and 73 close contacts. RESULTS: MA-ELA had an overall sensitivity and specificity of 61% (94/155)and 95% (200/211), respectively. We further examined whether the dissociation of immune complexes increases the number of positive reactions in thoseinitially found to be seronegative (SN). The subset of 112 (76 controls and 36 TB) MA-EIA SN samples tested using ICMA-EIA yielded an overall sensitivity and specificity of 83% and 100%. The ICMA-EIA results improved the overall sensitivity from 61 to 80% without changing specificity. CONCLUSION: These preliminary results suggest that MA-EIA followed by ICMA-EIA, for SN samples, might serve as a fast, cheap, and easy method for thediagnosis of TB in less than 48 hours.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:31:08