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Titolo:
Characterization of Porphyromonas gingivalis-induced degradation of epithelial cell junctional complexes
Autore:
Katz, J; Sambandam, V; Wu, JH; Michalek, SM; Balkovetz, DF;
Indirizzi:
Univ Alabama, Dept Microbiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Microbiol, Birmingham, AL 35294 USA Univ Alabama, Dept Oral Biol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Oral Biol, Birmingham, AL 35294 USA Univ Alabama, Dept Med, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 , Dept Med, Birmingham, AL 35294 USA Univ Alabama, Dept Cell Biol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Cell Biol, Birmingham, AL 35294 USA Vet Adm Med Ctr, Birmingham, AL 35233 USA Vet Adm Med Ctr Birmingham AL USA 35233 Med Ctr, Birmingham, AL 35233 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 3, volume: 68, anno: 2000,
pagine: 1441 - 1449
SICI:
0019-9567(200003)68:3<1441:COPGDO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTEROIDES-GINGIVALIS; CYSTEINE PROTEINASE; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; MAMMALIAN-CELLS; ARG-GINGIPAIN; E-CADHERIN; LYS-GINGIPAIN; INVASION; PURIFICATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
66
Recensione:
Indirizzi per estratti:
Indirizzo: Katz, J Univ Alabama, Dept Microbiol, 258 Bevill Biomed Res Bldg,845 19th St S, Birmingham, AL 35294 USA Univ Alabama 258 Bevill Biomed Res Bldg,845 19th St S Birmingham AL USA 35294
Citazione:
J. Katz et al., "Characterization of Porphyromonas gingivalis-induced degradation of epithelial cell junctional complexes", INFEC IMMUN, 68(3), 2000, pp. 1441-1449

Abstract

Porphyromonas gingivalis is considered among the etiological agents of human adult periodontitis, Although in vitro studies have shown that P. gingivalis has the ability to invade epithelial cell lines, its effect on the epithelial barrier junctions is not known. Immunofluorescence analysis of human gingival epithelial cells confirmed the presence of tight-junction (occludin), adherens junction (E-cadherin), and cell-extracellular matrix junction (pl-integrin) transmembrane proteins. These transmembrane proteins are expressed in Madin-Darby canine kidney (MDCK) cells, In addition, MDCK cells polarize and therefore serve as a useful in vitro model for studies on the epithelial cell barrier. Using the MDCK cell system, we examined the effectof P. gingivalis on epithelial barrier function, Exposure of the basolateral surfaces of MDCK cells to P. gingivalis (>10(9) bacteria/ml) resulted ina decrease in transepithelial resistance. Immunofluorescence microscopy demonstrated decreases in the amounts of immunoreactive occludin, E-cadherin,and pl-integrin at specific times which were related to a disruption of cell-cell junctions in MDCK cells exposed to basolateral P. gingivalis. Disruption of cell-cell junctions was also observed upon apical exposure to bacteria; however, the effects took longer than those seen upon basolateral exposure. Cell viability was not affected by either basolateral or apical exposure to P. gingivalis, Western blot analysis demonstrated hydrolysis of occludin, E-cadherin, and pl-integrin in lysates derived from MDCK cells exposed to P. gingivalis. Immunoprecipitated occludin and E-cadherin molecules from MDCK cell lysates were also degraded by P. gingivalis, suggesting a bacterial protease(s) capable of cleaving these epithelial junction transmembrane proteins, Collectively, these data suggest that P. gingivalis is able to invade the deeper structures of connective tissues via a paracellular pathway by degrading epithelial cell-cell junction complexes, thus allowing the spread of the bacterium, These results also indicate the importance of a critical threshold concentration of P. gingivalis to initiate epithelial barrier destruction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:55:31