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Titolo:
Additional transduction events after subretinal readministration of recombinant adeno-associated virus
Autore:
Anand, V; Chirmule, N; Fersh, M; Maguire, AM; Bennett, J;
Indirizzi:
Univ Penn, Scheie Eye Inst, Dept Ophthalmol, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 phthalmol, Philadelphia, PA 19104 USA Univ Penn, Inst Human Gene Therapy, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 e Therapy, Philadelphia, PA 19104 USA
Titolo Testata:
HUMAN GENE THERAPY
fascicolo: 3, volume: 11, anno: 2000,
pagine: 449 - 457
SICI:
1043-0342(20000210)11:3<449:ATEASR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIATED GENE-TRANSFER; ADENOASSOCIATED VIRUS; IN-VIVO; RETINAL DEGENERATION; TRANSGENE EXPRESSION; IMMUNE-RESPONSE; VIRAL VECTORS; ALLOGRAFTS; THERAPY; RESCUE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Bennett, J Univ Penn, Scheie Eye Inst, Dept Ophthalmol, 51 N 39th St, Philadelphia, PA 19104 USA Univ Penn 51 N 39th St Philadelphia PA USA 19104 , PA 19104 USA
Citazione:
V. Anand et al., "Additional transduction events after subretinal readministration of recombinant adeno-associated virus", HUM GENE TH, 11(3), 2000, pp. 449-457

Abstract

Subretinal delivery of recombinant adeno-associated virus (rAAV) results in a systemic humoral response in the adult immunocompetent mouse. We characterized this response end determined whether it is possible to readministerrAAV to the subretinal space despite the presence of antibodies to the vector, A systemic humoral response to rAAV capsid proteins was induced by either unilateral subretinal injection or by intradermal administration of 1 x10(9) infectious units of rAAV carrying the cDNA encoding green fluorescent protein (GFP), rAAV-GFP, Experiments were performed in cohorts of adult C57BL/6 mice, Assessment of systemic humoral response to viral capsid protein:was performed through enzyme-linked immunoabsorbent assay (ELISA) and infection inhibition studies of serum samples 3 weeks after virus delivery, The rAAV-GFP virus was readministered by subretinal injection, GFP expressionafter subretinal administration was evaluated ophthalmoscopically throughout the course of: the experiment and histologically at the termination of the experiment. We observed significant systemic humoral responses to viral capsid protein after subretinal delivery of rAAV. Intradermal injection resulted in a-larger humoral response (with a higher percentage of neutralizing antibodies) than subretinal injection. Additional transduction events were observed after readministration of rAAV despite the presence of strong humoral response to the vector.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:21:44