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Titolo:
Sera from children with Type 1 diabetes mellitus react against a new groupof antigens composed of lysophospholipids
Autore:
Bleich, D; Polak, M; Chen, SY; Swiderek, KM; Levy-Marchal, C;
Indirizzi:
Hop Robert Debre, U457, INSERM, Div Endocrinol & Diabet, F-75019 Paris, France Hop Robert Debre Paris France F-75019 ol & Diabet, F-75019 Paris, France Gonda Res Ctr, Div Endocrinol Diabet & Metab, Duarte, CA USA Gonda Res Ctr Duarte CA USA iv Endocrinol Diabet & Metab, Duarte, CA USA City Hope Natl Med Ctr, Beckman Res Inst, Duarte, CA 91010 USA City Hope Natl Med Ctr Duarte CA USA 91010 Res Inst, Duarte, CA 91010 USA
Titolo Testata:
HORMONE RESEARCH
fascicolo: 2, volume: 52, anno: 1999,
pagine: 86 - 94
SICI:
0301-0163(199908)52:2<86:SFCWT1>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISLET-CELL ANTIBODIES; GLUTAMIC-ACID DECARBOXYLASE; IDDM; SULFATIDE; GANGLIOSIDES; PREDICTION; RISK; SENSITIVITY; AUTOANTIGEN; IATROBEADS;
Keywords:
Type 1diabetes; lysophospholipids; islet cell antibodies; immunoassay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Polak, M Hop Robert Debre, U457, INSERM, Div Endocrinol & Diabet, 48 Blvd Serurier,F-75019 Paris, France Hop Robert Debre 48 Blvd Serurier Paris France F-75019 s, France
Citazione:
D. Bleich et al., "Sera from children with Type 1 diabetes mellitus react against a new groupof antigens composed of lysophospholipids", HORMONE RES, 52(2), 1999, pp. 86-94

Abstract

Several autoantibodies related to Type 1 diabetes mellitus and their corresponding autoantigens have been previously identified. While peptide antigens are more widely recognized, lipid antigens like sulfatides and gangliosides are also known epitopes for the diabetic humoral immune response. Isletcell antibodies (ICA) in Type 1 diabetes are heterogeneous immunoglobulinsdirected against selected antigens in the islets of Langerhans, Moreover, ICA may be the best predictive marker of disease in family members of patients with Type 1 diabetes. The aims of this study were: (1) to purify lipidsfrom porcine pancreas that contain ICA epitopes; (2) to characterize theselipid antigens, and (3) to use the purified lipids in an assay to detect antibodies in patients with Type 1 diabetes. A unique family of 4 lysophospho- lipids, 1 fully characterized as lysophosphatidylmyoinositol, partially inhibited ICA staining, and therefore, were considered to be candidate antigens for an ICA immunoassay. Using a dot blot immunoassay, we detected antibodies directed against these phospholipids in 28 out of 46 (61%) diabetic sera, while detecting only 1 false positive out of 28 nondiabetic sera (3.6%; p < 0.0001 comparing diabetic vs. nondiabetic serum). Therefore, lysophospholipid immunoassay positivity is present in sera of Type 1 diabetic patients. Furthermore, we detected 15 out of 23 ICA-negative diabetic sera (65.2%), showing that our phospholipid immunoassay does not correlate with ICA positivity. Copyright (C) 2000 S. Karger AG, Basel.

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Documento generato il 30/11/20 alle ore 07:21:30