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Titolo:
Improved safety and titre of murine leukaemia virus (MLV)-based retroviralvectors
Autore:
Zhao, Y; Low, W; Collins, MKL;
Indirizzi:
Dept Immunol, London W1P 6DB, England Dept Immunol London England W1P 6DB ept Immunol, London W1P 6DB, England
Titolo Testata:
GENE THERAPY
fascicolo: 4, volume: 7, anno: 2000,
pagine: 300 - 305
SICI:
0969-7128(200002)7:4<300:ISATOM>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONSTITUTIVE TRANSPORT ELEMENT; MESSENGER-RNA EXPORT; POSTTRANSCRIPTIONAL REGULATION; NUCLEAR EXPORT; UNSPLICED RNA; TYPE-1; EXPRESSION; PROMOTES; SIGNAL; IDENTIFICATION;
Keywords:
retrovirus; retroviral vector; RNA packaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Collins, MKL Dept Immunol, Windeyer Bldg,46 Cleveland St, London W1P 6DB, England Dept Immunol Windeyer Bldg,46 Cleveland St London England W1P 6DB
Citazione:
Y. Zhao et al., "Improved safety and titre of murine leukaemia virus (MLV)-based retroviralvectors", GENE THER, 7(4), 2000, pp. 300-305

Abstract

Many retroviral vectors based on murine leukaemia virus (MLV) contain the first 420 nucleotides of the gag gene, as this was reported to increase vector titre by increasing the efficiency of RNA packaging. In this study, deletion of this gag sequence from ifs original location did not decrease the titre of two retroviral vectors, pBabe pure and MFG-S-. The two vectors could be improved by replacing the gag sequence with a CTE from Mason-Pfizer monkey virus (MPMV). This substitution improved vector titre, while eliminating a region of homology between vector and packaging constructs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 10:47:44