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Titolo:
Intrascleral concentration vs depth profile of mitomycin-C after episcleral application: Impact of irrigation
Autore:
Vass, C; Georgopoulos, M; El Menyawi, I; Radda, S; Nimmerrichter, P; Menapace, R;
Indirizzi:
Univ Vienna, Dept Ophthalmol, Vienna, Austria Univ Vienna Vienna Austria niv Vienna, Dept Ophthalmol, Vienna, Austria Univ Vienna, Dept Internal Med, Vienna, Austria Univ Vienna Vienna Austria v Vienna, Dept Internal Med, Vienna, Austria
Titolo Testata:
EXPERIMENTAL EYE RESEARCH
fascicolo: 2, volume: 70, anno: 2000,
pagine: 139 - 143
SICI:
0014-4835(200002)70:2<139:ICVDPO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLAUCOMA FILTRATION SURGERY; POSTOPERATIVE 5-FLUOROURACIL; INTRAOPERATIVE MITOMYCIN; EXTRAOCULAR APPLICATION; ADJUNCTIVE MITOMYCIN; REFRACTORY GLAUCOMA; FILTERING SURGERY; CILIARY BODY; RABBIT MODEL; TRABECULECTOMY;
Keywords:
glaucoma; mitomycin-C; filtering surgery; complication; toxicity; ciliary body;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Vass, C Univ Vienna, Dept Ophthalmol, Waehringer Guertel 18, Vienna, Austria Univ Vienna Waehringer Guertel 18 Vienna Austria Vienna, Austria
Citazione:
C. Vass et al., "Intrascleral concentration vs depth profile of mitomycin-C after episcleral application: Impact of irrigation", EXP EYE RES, 70(2), 2000, pp. 139-143

Abstract

Mitomycin-C has been reported to cause toxic effects on the ciliary body after episcleral application during glaucoma surgery, We investigated the intrascleral diffusion of mitomycin-C in an experimental model. The episcleral sides of scleral quadrants of 14 human donor eyes were exposed for 5 min to sponges (corneal light shield, Merocel corp., Mystic, CT, U.S.A.) soakedwith 200 mu g ml(-1) mitomycin-C. After the exposure one of four quadrantswas not irrigated and the episcleral sides of three quadrants were irrigated with 40, 100 and 200 ml saline. A 9 mm scleral disk was punched out witha trephine and frozen on a kryotome plate 2 min after the end of mitomycin-C exposure. An 8 mm diameter scleral disk was then cut with a trephine, again frozen on a krgotome plate and then horizontally dissected with a kryotome. For analysis purposes seven cuts of 20 mu m thickness were combined toone layer of 140 mu m. Six layers could be reproduced and were analysed. The mitomycin-C concentrations of these layers were analysed by high-performance liquid chromatography. A concentration vs depth profile was calculatedfor each group, and the half-width of concentration was calculated by log-linear regression. The mitomycin-C concentration of layer 1 was 24.51 mu g g(-1) (+/-7.52) without irrigation, 13.15 mu g g(-1) (+/- 4.38) after 40 ml irrigation, 10.29(+/-3.53) after 100 ml irrigation and 8.4 mu g g(-1) (+/-1.62) after 200 ml irrigation. In layers 1-3 the concentration of mitomycin-C was significantly reduced by irrigation (ANOVA). In the deeper intrascleral layers irrigation had no effect on the mitomycin-C concentrations. Between layers 2 and 6 the half-width of the mitomycin-C concentration was 101 mu m (no-irrigation group), 141 mu m (40 ml irrigation group), 153 mu m (100 ml irrigation group), and 164 mu m (200 ml irrigation group). Irrigation reduced the mitomycin-C concentration only down to half of the scleral thickness, leaving the deep intrascleral concentrations unchanged. (C) 2000 Academic Press.

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Documento generato il 30/11/20 alle ore 07:13:08