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Titolo:
Cultured tumor cells of murine submandibular gland origin: a model to investigate pH(i) regulation of salivary cells
Autore:
Trzaskawka, E; Vigo, J; Egea, JC; Goldsmith, MC; Salmon, JM; De Periere, DD;
Indirizzi:
Univ Perpignan, UPRES Biol Physicochim Syst Integres 1947, Lab PhysicochemBiol Integrated Syst, F-66860 Perpignan, France Univ Perpignan Perpignan France F-66860 Syst, F-66860 Perpignan, France Univ Montpellier 1, Physiol Lab, Fac Odontol, Montpellier, France Univ Montpellier 1 Montpellier France Fac Odontol, Montpellier, France
Titolo Testata:
EUROPEAN JOURNAL OF ORAL SCIENCES
fascicolo: 1, volume: 108, anno: 2000,
pagine: 54 - 58
SICI:
0909-8836(200002)108:1<54:CTCOMS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PAROTID ACINAR-CELLS; INTRACELLULAR PH; NA+/H+ EXCHANGE; MUSCARINIC STIMULATION; INDUCED ACTIVATION; RAT; SECRETION; CA2+; FLUOROPROBE; TRANSPORT;
Keywords:
salivary glands; intracellular pH; Na+/H+ antiport; Cl-/HCO3- antiport; Na+/K+/2Cl(-) symport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Trzaskawka, E Univ Perpignan, UPRES Biol Physicochim Syst Integres 1947, Lab PhysicochemBiol Integrated Syst, 52 Ave Villeneuve, F-66860 Perpignan, France Univ Perpignan 52 Ave Villeneuve Perpignan France F-66860 e
Citazione:
E. Trzaskawka et al., "Cultured tumor cells of murine submandibular gland origin: a model to investigate pH(i) regulation of salivary cells", EUR J OR SC, 108(1), 2000, pp. 54-58

Abstract

Intracellular pH (pH(i)) and several ion transport mechanisms in cultured murine salivary gland cells (SCA-9) were studied using a videomicrofluorometric method and the H+-specific probe C-SNARF-1. The aim of this study was to test SCA-9 cells' pHi regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pHi in unstimulated cells was estimated to be 7.17+/-0.07. To investigate the presence of Na+/H+ and Cl-/HCO3- antiports as well as Na+/K+/2Cl(-) symports in SCA-9 cells, we used different specific blockers, dimethyl-amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA-9 cell capacity toregulate their pHi in response to alkaline and acid loads, we applied the NH4Cl prepulse method to all these blockers. The results showed that SCA-9 cells possess both antiports and symports involved in pHi regulation, and that this cell line can be used as a convenient model to study pHi regulatory mechanisms in salivary cells.

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Documento generato il 23/01/20 alle ore 18:31:04