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Titolo:
Metabotropic glutamate receptors and blockade of glial Krebs cycle depressglycinergic synaptic currents of mouse hypoglossal motoneurons
Autore:
Hulsmann, S; Oku, Y; Zhang, WQ; Richter, DW;
Indirizzi:
Univ Gottingen, Zentrum Physiol & Pathophysiol, Abt Neuro & Sinnesphysiol,D-37073 Gottingen, Germany Univ Gottingen Gottingen Germany D-37073 siol,D-37073 Gottingen, Germany Kyoto Univ, Inst Frontier Med Sci, Dept Med Syst Control, Sakyo Ku, Kyoto 6068397, Japan Kyoto Univ Kyoto Japan 6068397 t Control, Sakyo Ku, Kyoto 6068397, Japan
Titolo Testata:
EUROPEAN JOURNAL OF NEUROSCIENCE
fascicolo: 1, volume: 12, anno: 2000,
pagine: 239 - 246
SICI:
0953-816X(200001)12:1<239:MGRABO>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYOTROPHIC-LATERAL-SCLEROSIS; RAT-BRAIN-STEM; PRESYNAPTIC DEPRESSION; HIPPOCAMPAL-NEURONS; GLUCOSE DEPRIVATION; SPINAL-CORD; IN-VITRO; TRANSMISSION; ACTIVATION; FLUOROCITRATE;
Keywords:
glutamate transport; L-AP4; monofluoroacetic acid; synaptic transmission; t-ACPD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Hulsmann, S Univ Gottingen, Zentrum Physiol & Pathophysiol, Abt Neuro & Sinnesphysiol,Humboldtallee 23, D-37073 Gottingen, Germany Univ Gottingen Humboldtallee 23 Gottingen Germany D-37073 any
Citazione:
S. Hulsmann et al., "Metabotropic glutamate receptors and blockade of glial Krebs cycle depressglycinergic synaptic currents of mouse hypoglossal motoneurons", EUR J NEURO, 12(1), 2000, pp. 239-246

Abstract

Metabotropic glutamate receptors are known to depress synaptic transmission by inhibiting transmitter release from presynaptic nerve terminals. This study reports the effects of presynaptic metabotropic glutamate receptor activation on inhibitory synaptic transmission in hypoglossal motoneurons in brainstem slice preparations of neonatal mice. Whole-cell patch-clamp recordings were performed on hypoglossal motoneurons of 2-6-day-old mice. Monosynaptic glycinergic currents were elicited by electrical stimulation of the nucleus of Roller. Application of the specific metabotropic glutamate receptor agonists (+/-)-1-aminocyclopentane-trans-1,3,dicarboxylic acid (t-ACPD), (2s,2'R,3'R)-2-(2',3'-dicarboxylcyclopropyl)-glycine (DCG-IV) or L-2-amino-4-phosphonobutyric acid (L-AP4) depressed stimulus-evoked glycinergic inhibitory postsynaptic currents (IPSCs) by an average of 39.5, 59.4 and 39.2%, respectively. In the presence of t-ACPD, glycinergic miniature IPSCs werereduced in frequency but not in amplitude, which is indicative of a presynaptic mechanism. A similar reduction of IPSC amplitude was observed in the presence of elevated extracellular glutamate or during application of D,L-threo-hydroxyaspartate (THA), a blocker of glutamate transport, respectively. The data suggest that uptake of glutamate, which is predominately carriedout by glial cells, can prevent spill-over of glutamate and activation of metabotropic glutamate receptors. A reduction of IPSCs was also observed following application of monofluoroacetic acid, a substance acting specifically on glial cells. Our results suggest that glial regulation of extracellular glutamate uptake can prevent spillover of glutamate, and glutamatergic depression of glycinergic inhibition in hypoglossal motoneurons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 06:18:30