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Titolo:
CD3 activation induces concentrative nucleoside transport in human T lymphocytes
Autore:
Kichenin, K; Pignede, G; Fudalej, F; Seman, M;
Indirizzi:
Univ Paris 07, Grp Immunol Denis Diderot, F-75251 Paris 05, France Univ Paris 07 Paris France 05 ol Denis Diderot, F-75251 Paris 05, France Labs Mayoly Spindler, Chatou, France Labs Mayoly Spindler Chatou FranceLabs Mayoly Spindler, Chatou, France
Titolo Testata:
EUROPEAN JOURNAL OF IMMUNOLOGY
fascicolo: 2, volume: 30, anno: 2000,
pagine: 366 - 370
SICI:
0014-2980(200002)30:2<366:CAICNT>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROLIFERATIVE RATE; CELLS; THYMOCYTES; ADENOSINE; LYMPHOMA; CLONING;
Keywords:
nucleoside transporter; T lymphocyte; CD3 activation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Seman, M Univ Paris 07, Grp Immunol Denis Diderot, Hall Biotechnol,Tour 54,CP7124,2Pl Jussieu, F-75251 Paris 05, France Univ Paris 07 Hall Biotechnol,Tour 54,CP7124,2 Pl Jussieu Paris France 05
Citazione:
K. Kichenin et al., "CD3 activation induces concentrative nucleoside transport in human T lymphocytes", EUR J IMMUN, 30(2), 2000, pp. 366-370

Abstract

Nucleoside transport, assessed by measuring deoxythymidine influx, was investigated in normal and CD3-activated human peripheral blood mononuclear cells (PBMC) and in the CEM cell line. On both cell types, an equilibrative nitrobenzylmercaptopurine (NBMPR)-sensitive (es) transporter encoded by the hENT(1) gene was identified on vesting cells, although the expression levelwas about 20-fold higher on CEM cells than on resting peripheral T lymphocytes. After stimulation with anti-CD3, a strong increase of nucleoside transport was observed in PBMC accompanied by a mild augmentation of NBMPR binding sites on the, cell surface. Most of this improved transport capacity was NBMPR insensitive, dependent on Na+ concentration in the medium, and displayed the features of a concentrative process. Similar results were obtained with CEM cells despite their high basal es level, indicating that the induction of a concentrative process for nucleoside salvage is a specific metabolic response associated with antigen-driven stimulation. In CEM cells, this induction did not affect the growth rate. The concentrative transporter involved does not correspond to any of those which have been cloned so far. Molecular characterization of this transporter should provide a new markerof antigen stimulation and will allow to define whether activation of the corresponding gene is under the control of TCR-CD3-induced second messengers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:56:42