Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Development and utilization of primary hepatocyte culture systems to evaluate metabolism, dna binding, and dna repair of xenobiotics
Autore:
Casciano, DA;
Indirizzi:
Natl Ctr Toxicol Res, Div Genet & Reprod Toxicol, Jefferson, AR 72079 USA Natl Ctr Toxicol Res Jefferson AR USA 72079 icol, Jefferson, AR 72079 USA Univ Arkansas Med Sci, Dept Pharmacol & Toxicol, Little Rock, AR 72205 USAUniv Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA Univ Arkansas Med Sci, Dept Biochem & Mol Biol, Little Rock, AR 72205 USA Univ Arkansas Med Sci Little Rock AR USA 72205 Little Rock, AR 72205 USA
Titolo Testata:
DRUG METABOLISM REVIEWS
fascicolo: 1, volume: 32, anno: 2000,
pagine: 1 - 13
SICI:
0360-2532(2000)32:1<1:DAUOPH>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY RAT HEPATOCYTES; CALORIC RESTRICTION; CHEMICAL CARCINOGENS; FISCHER-344 RATS; CELL-CULTURES; ASSAY; ADDUCTS; 6-NITROCHRYSENE; IDENTIFICATION; METHAPYRILENE;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Casciano, DA Natl Ctr Toxicol Res, Div Genet & Reprod Toxicol, Jefferson, AR 72079 USA Natl Ctr Toxicol Res Jefferson AR USA 72079 on, AR 72079 USA
Citazione:
D.A. Casciano, "Development and utilization of primary hepatocyte culture systems to evaluate metabolism, dna binding, and dna repair of xenobiotics", DRUG METAB, 32(1), 2000, pp. 1-13

Abstract

The use of isolated hepatocytes as an approach to evaluate hepatotoxic andhepatocarcinogenic compounds and investigate mechanisms by which chemicalsinduce liver lesions is well established. This review discusses techniquesdeveloped in the author's laboratory describing (1) isolation and primary culture of rodent hepatocytes detailing methods which are optimal for obtaining large numbers of viable cells, (2) DNA damage induced by physical and chemical agents in rodent hepatocytes measured as unscheduled DNA synthesis, and (3) metabolic activation of model hepatocarcinogens, their binding toDNA, and identification of individual adducts thought to be responsible for induction of DNA repair.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 00:02:41