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Titolo:
Id-2 regulates critical aspects of human cytotrophoblast differentiation, invasion and migration
Autore:
Janatpour, MJ; McMaster, MT; Genbacev, O; Zhou, Y; Dong, JY; Cross, JC; Israel, MA; Fisher, SJ;
Indirizzi:
Univ Calif San Francisco, Grad Program Biomed Sci, Dept Stomatol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Grad Program Biomed Sci, Dept Lab Med, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Grad Program Biomed Sci, Dept Pharmaceut Chem, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Grad Program Biomed Sci, Dept Anat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Grad Program Biomed Sci, Dept Obstet Gynecol & Reprod Sci, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Neurol Surg, Brain Tumor Res Ctr, Preuss Lab, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Pediat, Brain Tumor Res Ctr, Preuss Lab, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Toronto, ON M5G 1X5, Canada Mt Sinai Hosp Toronto ON Canada M5G 1X5 Inst, Toronto, ON M5G 1X5, Canada Univ Toronto, Dept Mol & Med Genet, Toronto, ON, Canada Univ Toronto Toronto ON Canada Dept Mol & Med Genet, Toronto, ON, Canada Univ Toronto, Dept Obstet & Gynecol, Toronto, ON, Canada Univ Toronto Toronto ON Canada ept Obstet & Gynecol, Toronto, ON, Canada
Titolo Testata:
DEVELOPMENT
fascicolo: 3, volume: 127, anno: 2000,
pagine: 549 - 558
SICI:
0950-1991(200002)127:3<549:IRCAOH>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BHLH TRANSCRIPTION FACTOR; HLA-G; PLACENTAL CYTOTROPHOBLASTS; ENDOVASCULAR INVASION; ADHESION RECEPTORS; MOUSE GASTRULATION; DNA-BINDING; TROPHOBLAST; EXPRESSION; PREECLAMPSIA;
Keywords:
Id; cytotrophoblast; placenta; differentiation; integrin; cyclin; invasion; migration; human;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Fisher, SJ Univ Calif San Francisco, Grad Program Biomed Sci, Dept Stomatol, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 94143 USA
Citazione:
M.J. Janatpour et al., "Id-2 regulates critical aspects of human cytotrophoblast differentiation, invasion and migration", DEVELOPMENT, 127(3), 2000, pp. 549-558

Abstract

During early human placental development, the conceptus attaches itself tothe uterus through cytotrophoblast invasion. Invasive cytotrophoblast cells differentiate from precursor villous cytotrophoblasts, but the essential regulating factors in this process are unknown. Basic helix-loop-helix (bHLH) transcription factor dimers are essential regulators of mouse trophoblast development. We therefore examined the importance of this family of factors in the human placenta. In many cell lineages, bHLH factors are sequestered by members of the Id family, HLH proteins that lack the basic DNA binding domain (Inhibitor of DNA binding proteins (Id-l to Id-4)), During differentiation of some tissues, Id expression declines, allowing bHLH factors to dimerize, bind DNA and trans-activate lineage-specific genes, To begin to study the role of bHLH transcription factors in human placental development,we first characterized Id expression in cytotrophoblast cells. The cells expressed Id-3 constitutively; Id-2 was downregulated, at the mRNA and protein levels, as the cells differentiated in culture and in situ, respectively. In cases when cytotrophoblast differentiation was compromised tin placentas from women with preeclampsia, or in cells grown under hypoxic conditionsin culture), Id-2 expression was maintained. To assess the functional relevance of these correlations, we used an adenovirus vector to maintain Id-2 protein expression in cultured cytotrophoblasts, Compared to control (lacZ-expressing) cells, cytotrophoblasts transduced to constitutively express Id-2 retained characteristics of undifferentiated cells: alpha 1 integrin expression was low and cyclin B expression was retained. Furthermore, invasionthrough Matrigel was partially inhibited and migration was strikingly enhanced in Id-2-expressing cells. These results suggest that Id-2 and the bHLHfactors that it partners play important roles in human cytotrophoblast development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 06:02:25