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Titolo:
Role of the kinesin neck linker and catalytic core in microtubule-based motility
Autore:
Case, RB; Rice, S; Hart, CL; Ly, B; Vale, RD;
Indirizzi:
Univ Calif San Francisco, Dept Pharmacol, San Francisco, CA 94143 USA UnivCalif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Howard Hughes Med Inst, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
CURRENT BIOLOGY
fascicolo: 3, volume: 10, anno: 2000,
pagine: 157 - 160
SICI:
0960-9822(20000210)10:3<157:ROTKNL>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CRYSTAL-STRUCTURE; DIMERIC KINESIN; MOTOR DOMAIN; DIRECTION; NCD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Vale, RD Univ Calif San Francisco, Dept Pharmacol, 513 Parnassus Ave, San Francisco, CA 94143 USA Univ Calif San Francisco 513 Parnassus Ave San Francisco CA USA 94143
Citazione:
R.B. Case et al., "Role of the kinesin neck linker and catalytic core in microtubule-based motility", CURR BIOL, 10(3), 2000, pp. 157-160

Abstract

Kinesin motor proteins execute a variety of intracellular microtubule-based transport functions [1], Kinesin motor domains contain a catalytic core, which is conserved throughout the kinesin superfamily, followed by a neck region, which is conserved within subfamilies and has been implicated in controlling the direction of motion along a microtubule [2,3], Here, we have used mutational analysis to determine the functions of the catalytic core and the similar to 15 amino acid 'neck linker' (a sequence contained within the neck region) of human conventional kinesin, Replacement of the neck linker with a designed random coil resulted in a 200-500-fold decrease in microtubule velocity, although basal and microtubule-stimulated ATPase rates were within threefold of wildtype levels. The catalytic core of kinesin, without any additional kinesin sequence, displayed microtubule-stimulated ATPaseactivity, nucleotide-dependent microtubule binding, and very slow plus end-directed motor activity. On the basis of these results, we propose that the catalytic core is sufficient for allosteric regulation of microtubule binding and ATPase activity and that the kinesin neck linker functions as a mechanical amplifier for motion, Given that the neck linker undergoes a nucleotide-dependent conformational change [4], this region might act in an analogous fashion to the myosin converter, which amplifies small conformationalchanges in the myosin catalytic core [5,6].

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:16:13