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Titolo:
Viral-mediated gene delivery of constitutively activated G alpha s alters vasoreactivity
Autore:
Schutzer, WE; Watts, VJ; Chapman, J; Cumbay, MG; Neve, KA; Neve, RL; Mader, SL;
Indirizzi:
Portland VA Med Ctr, Res Serv, Portland, OR 97201 USA Portland VA Med CtrPortland OR USA 97201 es Serv, Portland, OR 97201 USA Oregon Hlth Sci Univ, Sch Med, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 ch Med, Portland, OR 97201 USA Purdue Univ, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Pharmacol, W Lafayette, IN 47907 USA Harvard Univ, Sch Med, Dept Genet, Belmont, MA 02178 USA Harvard Univ Belmont MA USA 02178 Med, Dept Genet, Belmont, MA 02178 USA
Titolo Testata:
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
fascicolo: 1-2, volume: 27, anno: 2000,
pagine: 9 - 13
SICI:
0305-1870(200001/02)27:1-2<9:VGDOCA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; RAT AORTA; PROTEIN; AGE; OVEREXPRESSION; EXPRESSION; DESENSITIZATION; MECHANISMS; RELAXATION; RESPONSES;
Keywords:
cAMP; gene transfer; G-protein; Herpes simplex virus; vascular tone; vasorelaxation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Mader, SL Portland VA Med Ctr, Res Serv, 3710 SW US Vet Hosp Rd, Portland,OR 97201 USA Portland VA Med Ctr 3710 SW US Vet Hosp Rd Portland OR USA 97201
Citazione:
W.E. Schutzer et al., "Viral-mediated gene delivery of constitutively activated G alpha s alters vasoreactivity", CLIN EXP PH, 27(1-2), 2000, pp. 9-13

Abstract

1. Decline in beta-adrenoceptor (beta-AR)-mediated function occurs with increasing age, as well as in multiple disease conditions. The mechanisms responsible for this decline include alterations in beta-AR itself, beta-AR coupling proteins, such as G-proteins, or other beta-AR-linked proteins, suchas G-protein receptor kinases and/or phosphatases.2. The present study examines the physiological effects of n vitro transfer of constitutively activated G alpha s (G alpha s-U227L) to both cultured vascular smooth muscle cells (VSMC) and whole aortic tissue of 6-month-old (adult) animals via a replication-deficient Herpes simplex virus (HSV) vector. These studies were conducted to provide a model for future examination of the role of G alpha s in the age-related decline in beta-AR-mediated vasorelaxation.3. Gene transfer was confirmed by western blotting for specific proteins. Aortic tissue infected with HSV-G alpha s-Q227L had reduced phenylephrine-induced contraction and enhanced isoproterenol-stimulated vasorelaxation, Infection of cultured VSMC with HSV-G alpha s-Q227L increased both basal- andisoproterenol-stimulated cAMP accumulation, whereas forskolin-stimulated cAMP production was unchanged.4. These results implicate G alpha s as a target for further investigationin age-related changes in vascular reactivity and support the use of viral-mediated gene transfer as an effective tool to study adrenergic signal transduction and physiology in vascular tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 02:36:11