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Titolo:
Viability of periosteal tissue obtained postmortem
Autore:
ODriscoll, SW; Meisami, B; Miura, Y; Fitzsimmons, JS;
Indirizzi:
Mayo Clin & Mayo Fdn, Cartilage & Connect Tissue Res Lab, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Lab, Rochester, MN 55905 USA
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 6, volume: 8, anno: 1999,
pagine: 611 - 616
SICI:
0963-6897(199911/12)8:6<611:VOPTOP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE DEFECTS; JOINT SURFACE-DEFECTS; OSTEOCHONDRAL DEFECTS; II COLLAGEN; IN-VITRO; GRAFTS; RECONSTRUCTION; REPAIR; NEOCHONDROGENESIS; CHONDROGENESIS;
Keywords:
articular cartilage repair; periosteum; chondrogenesis transplant; tissue engineering;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: O'Driscoll, SW Mayo Clin & Mayo Fdn, Cartilage & Connect Tissue Res Lab, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 , MN 55905 USA
Citazione:
S.W. O'Driscoll et al., "Viability of periosteal tissue obtained postmortem", CELL TRANSP, 8(6), 1999, pp. 611-616

Abstract

Periosteal autografts have the potential to regenerate articular cartilagedefects, but this potential is limited by the patients age. Allograft transplantation from a young donor to an older recipient might bypass this limitation. The effect of the time delay, between death and harvesting of a periosteal graft, on the chondrogenic potential of periosteum is important notonly for transplantation but also for studies dealing with tissues retrieved postmortem (i.e., including the periosteal explant model). The purpose of this study was to investigate the chondrogenic potential of periosteum obtained postmortem and a possible beneficial effect of hypothermia. Thirty NZ white rabbits (2 months old) were sacrificed and stored at room temperature or 4 degrees C for 0, 4, 6, 8, 12, 16, 18, or 24 h. Periosteal explants were then obtained and a standard cartilage yield assay performed by culturing them for 6 weeks using the periosteal organ culture model as previous published. TGF-beta 1 (10 ng/ml) was added for the first 14 days of culture. Histochemical analysis and quantitative collagen typing were performed. Inthe explants from the animals kept for 4 h at room temperature growth and chondrogenesis were dramatically reduced. Little or no chondrogenesis was seen in explants from rabbits maintained at room temperature after 4-8 h (ormore) postmortem. Cooling the rabbits to 4 degrees C partially prevented this loss of viability and continued to do so for 24 h. Even storage at 4 degrees C did not eliminate the decrease in chondrogenic potential, though itdid permit partial preservation of chondrogenic potential. if periosteum is to be used for allograft transplantation, or if it is used for experimental study, its viability must be assured. This is beat accomplished by harvesting it immediately postmortem. Preservation techniques, cryopreservation,or hypothermia might be useful in preserving periosteal chondrogenic potential.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 23:03:09