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Titolo:
The gene for human TATA-binding-protein-associated factor (TAF(II)) 170: structure, promoter and chromosomal localization
Autore:
van der Knaap, JA; van den Boom, V; Kuipers, J; van Eijk, MJT; van der Vliet, PC; Timmers, HTM;
Indirizzi:
Univ Utrecht, Physiol Chem Lab, NL-3508 TA Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TA 3508 TA Utrecht, Netherlands Univ Utrecht, Ctr Biomed Genet, NL-3508 TA Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TA 3508 TA Utrecht, Netherlands Univ Utrecht, Dept Immunol, NL-3508 TA Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TA 3508 TA Utrecht, Netherlands Wilhelmina Childrens Hosp, Lab Metab Dis, NL-3508 AB Utrecht, Netherlands Wilhelmina Childrens Hosp Utrecht Netherlands NL-3508 AB ht, Netherlands
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 345, anno: 2000,
parte:, 3
pagine: 521 - 527
SICI:
0264-6021(20000201)345:<521:TGFHTF>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; SACCHAROMYCES-CEREVISIAE; TBP BINDING; TRANSCRIPTION; EXPRESSION; YEAST; CLONING; MOT1; HOMOLOGY; SUBUNIT;
Keywords:
chromosome 10; RNA polymerase II; SNF2; TFIID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Timmers, HTM Univ Utrecht, Physiol Chem Lab, POB 80042, NL-3508 TA Utrecht, Netherlands Univ Utrecht POB 80042 Utrecht Netherlands NL-3508 TA rlands
Citazione:
J.A. van der Knaap et al., "The gene for human TATA-binding-protein-associated factor (TAF(II)) 170: structure, promoter and chromosomal localization", BIOCHEM J, 345, 2000, pp. 521-527

Abstract

The TATA-binding protein (TBP) plays a central role in eukaryotic transcription and forms protein complexes with TBP-associated factors (TAFs). The genes encoding TAF(II) proteins frequently map to chromosomal regions altered in human neoplasias. TAF(II)170 of B-TFIID is a member of the SF2 superfamily of putative helicases. Members of this superfamily have also been implicated in several human genetic disorders. In this study we have isolated human genomic clones encoding TAF(II)170 and we show that the gene contains 37 introns. Ribonuclease-protection experiments revealed that TAF(II)170 has multiple transcription start sites, consistent with the observation that the promoter lacks a canonical TATA box and initiator element. Deletion analysis of the promoter region showed that a fragment of 264 bp is sufficientto direct transcription. In addition, we determined the chromosomal localization by two independent methods which mapped the gene to human chromosome10q22-q23 between the markers D10S185 and WI-1183. The region surrounding these markers has been implicated in several human disorders.

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Documento generato il 30/11/20 alle ore 06:54:38