Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The role of microtubules in the regulation of proteoglycan synthesis in chondrocytes under hydrostatic pressure
Autore:
Jortikka, MO; Parkkinen, JJ; Inkinen, RI; Karner, J; Jarvelainen, HT; Nelimarkka, LO; Tammi, MI; Lammi, MJ;
Indirizzi:
Univ Kuopio, Dept Anat, FIN-70211 Kuopio, Finland Univ Kuopio Kuopio Finland FIN-70211 ept Anat, FIN-70211 Kuopio, Finland Univ Kuopio, Dept Pathol, FIN-70211 Kuopio, Finland Univ Kuopio Kuopio Finland FIN-70211 t Pathol, FIN-70211 Kuopio, Finland Univ Turku, Dept Med, SF-20500 Turku, Finland Univ Turku Turku Finland SF-20500 rku, Dept Med, SF-20500 Turku, Finland Univ Turku, Dept Med Biochem, SF-20500 Turku, Finland Univ Turku Turku Finland SF-20500 t Med Biochem, SF-20500 Turku, Finland Univ Tartu, Dept Zool, EE-50090 Tartu, Estonia Univ Tartu Tartu Estonia EE-50090 tu, Dept Zool, EE-50090 Tartu, Estonia
Titolo Testata:
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
fascicolo: 2, volume: 374, anno: 2000,
pagine: 172 - 180
SICI:
0003-9861(20000215)374:2<172:TROMIT>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARTICULAR-CARTILAGE; CHONDROITIN SULFATE; GOLGI-APPARATUS; MESSENGER-RNA; CULTURED CHONDROCYTES; ALTERED AGGRECAN; RUNNING EXERCISE; MATRIX SYNTHESIS; BREFELDIN-A; CELLS;
Keywords:
hydrostatic pressure; microtubules; cartilage proteoglycans; glycosylation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Lammi, MJ Univ Kuopio, Dept Anat, POB 1627, FIN-70211 Kuopio, Finland UnivKuopio POB 1627 Kuopio Finland FIN-70211 1 Kuopio, Finland
Citazione:
M.O. Jortikka et al., "The role of microtubules in the regulation of proteoglycan synthesis in chondrocytes under hydrostatic pressure", ARCH BIOCH, 374(2), 2000, pp. 172-180

Abstract

Chondrocytes of the articular cartilage sense mechanical factors associated with joint loading, such as hydrostatic pressure, and maintain the homeostasis of the extracellular matrix by regulating the metabolism of proteoglycans (PGs) and collagens. Intermittent hydrostatic pressure stimulates, while continuous high hydrostatic pressure inhibits, the biosynthesis of PGs. High continuous hydrostatic pressure also changes the structure of cytoskeleton and Golgi complex in cultured chondrocytes. Using microtubule (MT)-affecting drugs nocodazole and taxol as tools we examined whether MTs are involved in the regulation of PG synthesis in pressurized primary chondrocyte monolayer cultures. Disruption of the microtubular array by nocodazole inhibited [S-35]sulfate incorporation by 39-48%, while MT stabilization by taxolcaused maximally a 17% inhibition. Continuous hydrostatic pressure furtherdecreased the synthesis by 34-42% in nocodazole-treated cultures. This suggests that high pressure exerts its inhibitory effect through mechanisms independent of MTs. On the other hand, nocodazole and taxol both prevented the stimulation of PG synthesis by cyclic 0.5 Hz, 5 MPa hydrostatic pressure,The drugs did not affect the structural and functional properties of the PGs, and none of the treatments significantly affected cell viability, as indicated by the high level of PO synthesis 24-48 h after the release of drugs and/or high hydrostatic pressure. Our data on two-dimensional chondrocytecultures indicate that inhibition of PG synthesis by continuous high hydrostatic pressure does not interfere with the MT-dependent vesicle traffic, while the stimulation of synthesis by cyclic pressure does not occur if the dynamic nature of MTs is disturbed by nocodazole. Similar phenomena may operate in cartilage matrix embedded chondrocytes, (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 11:25:24