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Titolo:
Induced sputum cell counts in healthy adults
Autore:
Belda, J; Leigh, R; Parameswaran, K; OByrne, PM; Sears, MR; Hargreave, FE;
Indirizzi:
McMaster Univ, St Josephs Hosp, Dept Med, Firestone Reg Chest & Allergy Unit,Asthma Res Grp, Hamilton, ON L8N 4A6, Canada McMaster Univ Hamilton ON Canada L8N 4A6 rp, Hamilton, ON L8N 4A6, Canada
Titolo Testata:
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
fascicolo: 2, volume: 161, anno: 2000,
pagine: 475 - 478
SICI:
1073-449X(200002)161:2<475:ISCCIH>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
OBSTRUCTIVE PULMONARY-DISEASE; COMMUNITY-BASED POPULATION; SKIN-TEST REACTIVITY; AIRWAY INFLAMMATION; HYPERTONIC SALINE; ASTHMA; EOSINOPHILS; INDUCTION; BASOPHILS; INDEXES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Hargreave, FE McMaster Univ, St Josephs Hosp, Dept Med, Firestone Reg Chest & Allergy Unit,Asthma Res Grp, 50 Charlton Ave E, Hamilton, ON L8N 4A6, Canada McMaster Univ 50 Charlton Ave E Hamilton ON Canada L8N 4A6 a
Citazione:
J. Belda et al., "Induced sputum cell counts in healthy adults", AM J R CRIT, 161(2), 2000, pp. 475-478

Abstract

Induced sputum cell counts provide a relatively noninvasive method to evaluate the presence, type, and degree of inflammation in the airways of the lungs. Their interpretation requires a knowledge of normal values from a healthy population. The objective was to examine the total and differential cell counts in induced sputum from a sample of healthy adults. A total of 118healthy nonsmoking adults were studied. None had asthma or airflow obstruction (negative history, FEV1 greater than or equal to 80% predicted, ratio of FEV1 to vital capacity [FEV1/VC] greater than or equal to 80%, methacholine PC20 greater than or equal to 16 mg/ml). Forty-six were atopic. Sputum induction produced an adequate sample in 96 subjects [53 males, mean age (range) 36 (18 to 60) yr]. The expectorate was processed within 2 h; sputum was selected, treated with dithiothreitol, filtered, and examined in a hemocytometer for total cell count and viability and on Wright-stained cytospinsfor a differential cell count. The mean, median (90th percentile) total cell count was 4.1, 2.4 (9.7) x 10(6) cells/g and cell viability was 69.6, 72.0 (89.7)%. The proportions of eosinophils were 0.4, 0.0 (1.1)%, neutrophils 37.5, 36.7 (64.0)%, macrophages 58.8, 60.8 (86.1)%, lymphocytes 1.0, 0.5 (2.6)%, metachromatic cells 0.0, 0.0 (0.04)%, and bronchial epithelial cells 1.6, 0.3 (4.4)%, respectively. Female gender and atopy were associated with a significant elevation of eosinophils; mean difference between male/female was 0.3% (p = 0.043) and between atopic/nonatopic 0.4% (p = 0.024). This study has identified reference values for total and differential cell counts in induced sputum of healthy adults.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:10:25