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Titolo:
Ionizing radiation induces iNOS-mediated epithelial dysfunction in the absence of an inflammatory response
Autore:
Freeman, SL; MacNaughton, WK;
Indirizzi:
Univ Calgary, Dept Phys & Biophys, Calgary, AB T2N 4N1, Canada Univ Calgary Calgary AB Canada T2N 4N1 ophys, Calgary, AB T2N 4N1, Canada Univ Calgary, Gastrointestinal Res Grp, Calgary, AB T2N 4N1, Canada Univ Calgary Calgary AB Canada T2N 4N1 s Grp, Calgary, AB T2N 4N1, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
fascicolo: 2, volume: 278, anno: 2000,
pagine: G243 - G250
SICI:
0193-1857(200002)278:2<G243:IRIIED>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; EVOKED ELECTROLYTE TRANSPORT; GASTROINTESTINAL-TRACT; INTESTINAL FLUID; RABBIT ILEUM; RAT ILEUM; COLON; STIMULATION; THEOPHYLLINE; IRRADIATION;
Keywords:
radiotherapy; secretion; intestine; electrolyte transport; transgenic mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: MacNaughton, WK Univ Calgary, Dept Phys & Biophys, 3330 Hosp Dr NW, Calgary, AB T2N 4N1, Canada Univ Calgary 3330 Hosp Dr NW Calgary AB Canada T2N 4N1 ada
Citazione:
S.L. Freeman e W.K. MacNaughton, "Ionizing radiation induces iNOS-mediated epithelial dysfunction in the absence of an inflammatory response", AM J P-GAST, 278(2), 2000, pp. G243-G250

Abstract

Ionizing radiation induces intestinal epithelial hyporesponsiveness to secretagogues through an unknown mechanism. We investigated the role of the inducible isoform of nitric oxide (NO) synthase (iNOS)-derived NO in radiation-induced hyporesponsiveness. C57BL/6 mice were sham treated or exposed to 10-Gy gamma-radiation and were studied 3 days later. Tissues were mounted in Ussing-type diffusion chambers to assess chloride secretion in response to electrical field stimulation (EFS) and forskolin (10 mu M). Transport studies were also repeated in iNOS-deficient mice. White blood cell counts were significantly lower in irradiated mice, and there was no inflammatory response as shown by myeloperoxidase activity and histological assessment. iNOS mRNA levels and nitrate/nitrite concentrations were significantly elevated in irradiated colons. iNOS immunoreactivity localized to the epithelium. Colons from irradiated wild-type, but not iNOS-deficient, mice exhibited a significant reduction in the responsiveness of the tissue to EFS and forskolin. The hyporesponsiveness was reversed by L-N-6-(1-iminoethyl)lysine, 1400W, and dexamethasone treatments. iNOS-derived NO mediates colonic hyporesponsiveness 3 days after irradiation in the mouse in the absence of an inflammatory response.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/08/20 alle ore 23:20:50