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Titolo:
The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance and osmo-instigated gene expression changes in Saccharomyces cerevisiae
Autore:
Norbeck, J; Blomberg, A;
Indirizzi:
Univ Gothenburg, Dept Cell & Mol Biol, Lundberg Lab, SE-41390 Gothenburg, Sweden Univ Gothenburg Gothenburg Sweden SE-41390 , SE-41390 Gothenburg, Sweden
Titolo Testata:
YEAST
fascicolo: 2, volume: 16, anno: 2000,
pagine: 121 - 137
SICI:
0749-503X(20000130)16:2<121:TLOCPK>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION PATHWAY; IMMOBILIZED PH GRADIENT; RESPONSE ELEMENT STRE; 0.7 M NACL; YEAST GENE; S-CEREVISIAE; CYCLIC-AMP; GROWTH; CLONING; DEHYDROGENASE;
Keywords:
cAMP-dependent protein kinase; osmotic stress; protein expression; yeast;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Norbeck, J Univ Gothenburg, Dept Cell & Mol Biol, Lundberg Lab, Med Aregatan 9C, SE-41390 Gothenburg, Sweden Univ Gothenburg Med Aregatan 9C Gothenburg Sweden SE-41390 den
Citazione:
J. Norbeck e A. Blomberg, "The level of cAMP-dependent protein kinase A activity strongly affects osmotolerance and osmo-instigated gene expression changes in Saccharomyces cerevisiae", YEAST, 16(2), 2000, pp. 121-137

Abstract

The influence of cAMP-dependent protein kinase (PKA) on protein expressionduring exponential growth under osmotic stress was studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The responses of isogenicstrains (tpk2 Delta tpk3 Delta) with either constitutively low (tpk1(w1)),regulated (TPK1) or constitutively high (TPK1bcy1 Delta) PKA activity werecompared. The activity of cAMP-dependent protein kinase (PKA) was shown tobe a major determinant of osmotic shock tolerance. Proteins with increasedexpression during growth under sodium chloride stress could be grouped into three classes with respect to PKA activity, with the glycerol metabolic proteins GPD1, GPP2 and DAK1 standing out as independent of PKA. The other osmotically induced proteins displayed a variable dependence on PKA activity; fully PKA-dependent genes were TPS1 and GCY1, partly PKA-dependent genes were ENO1, TDH1, ALD3 and CTT1. The proteins repressed by osmotic stress also fell into distinct classes of PKA-dependency. Ymr116c was PKA-independent, while Pgi1p, Sam1p, Gdh1p and Vma1p were fully PKA-dependent. Hxk2p, Pdc1p, Ssb1p, Met6p, Atp2p and Hsp60p displayed a partially PKA-dependent repression. The promoters of all induced PKA-dependent genes have STRE sites intheir promoters suggestive of a mechanism acting via Msn2/4p. The mechanisms governing the expression of the other classes are unknown. From the protein expression data we conclude that a low PKA activity causes a protein expression resembling that of osmotically stressed cells, and furthermore makes cells tolerant to this type of stress. Copyright (C) 2000 John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 18:07:43