Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Replication ability in vitro and in vivo of equine infectious anemia virusavirulent Japanese strain
Autore:
Zheng, YH; Sentsui, H; Sugita, M; Nakaya, T; Kishi, M; Hagiwara, K; Inoshima, Y; Ishihara, C; Kono, Y; Lu, JL; Ikuta, K;
Indirizzi:
Hokkaido Univ, Inst Immunol Sci, Kita Ku, Sapporo, Hokkaido, Japan Hokkaido Univ Sapporo Hokkaido Japan , Kita Ku, Sapporo, Hokkaido, Japan Natl Inst Anim Hlth, Tsukuba, Ibaraki 305, Japan Natl Inst Anim Hlth Tsukuba Ibaraki Japan 305 Tsukuba, Ibaraki 305, Japan Rakuno Gakuen Univ, Sch Vet Med, Ebetsu, Hokkaido 069, Japan Rakuno GakuenUniv Ebetsu Hokkaido Japan 069 Ebetsu, Hokkaido 069, Japan Tokyo Univ Agr & Technol, Fac Agr, Tokyo 156, Japan Tokyo Univ Agr & Technol Tokyo Japan 156 hnol, Fac Agr, Tokyo 156, Japan Chinese Acad Agr Sci, Vet Res Inst, Key Lab Vet Biotechnol, Harbin, Peoples R China Chinese Acad Agr Sci Harbin Peoples R China ol, Harbin, Peoples R China Osaka Univ, Microbial Dis Res Inst, Dept Virol, Suita, Osaka 565, Japan Osaka Univ Suita Osaka Japan 565 nst, Dept Virol, Suita, Osaka 565, Japan
Titolo Testata:
VIROLOGY
fascicolo: 1, volume: 266, anno: 2000,
pagine: 129 - 139
SICI:
0042-6822(20000105)266:1<129:RAIVAI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG TERMINAL REPEAT; MOLECULAR CLONES; FEBRILE EPISODES; BLOOD MONOCYTES; HORSES; DISEASE; DNA; TAT; IDENTIFICATION; INVITRO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Ikuta, K 3-1 Yamadaoka, Suita, Osaka 5650871, Japan 3-1 Yamadaoka Suita Osaka Japan 5650871 ta, Osaka 5650871, Japan
Citazione:
Y.H. Zheng et al., "Replication ability in vitro and in vivo of equine infectious anemia virusavirulent Japanese strain", VIROLOGY, 266(1), 2000, pp. 129-139

Abstract

An attenuated equine infectious anemia virus (EIAV), V26, was previously prepared by 50 passages of the Japanese virulent strain V70 in primary horsemacrophage culture. The horses inoculated with this V26 virus were shown to raise neutralizing antibodies against V70 without any viremia. Here, we investigated the in vitro and in vivo replication ability of V26. Comparisonof the long-terminal repeat (LTR) sequences between V26 and V70 revealed alarge insertion within the LTR U3 hypervariable region of V26. V26 with the mutation in the LTR showed much higher promoter activity in vitro than V70. This is consistent with the much higher replication rate of V26 in horseprimary macrophage cultures compared with V70. In sharp contrast, we failed to identify the V26-specific LTR sequence by PCR, at least in sequential samples of plasma or peripheral blood mononuclear calls derived from three horses until day 62 after V26 inoculation. In contrast, antibody responses to EIAV were observed in all horses. The results suggest that the replication ability of V26 in vivo is extremely low. When one of the horses was subsequently challenged with cell-associated V70, it was found that the horse became PCR positive for EIAV. There was no LTR mutation in EIAV genome in samples periodically prepared from the V70-challenged horse. Thus it was suggested that the LTR mutation in EIAV, which occurs during serial passage in vitro, affects EIAV replication in vitro and in vivo. (C) 2000 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 07:29:10