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Titolo:
Cloning, sequencing, and analysis of cDNA encoding bovine granulocyte-colony stimulating factor
Autore:
Heidari, M; Kehrli, ME;
Indirizzi:
Iowa State Univ, Dept Microbiol Immunol & Prevent Med, Ames, IA 50010 USA Iowa State Univ Ames IA USA 50010 munol & Prevent Med, Ames, IA 50010 USA USDA ARS, Natl Anim Dis Ctr, Metab Dis & Immunol Res Unit, Ames, IA 50010 USA USDA ARS Ames IA USA 50010 tab Dis & Immunol Res Unit, Ames, IA 50010 USA
Titolo Testata:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
fascicolo: 2, volume: 73, anno: 2000,
pagine: 183 - 191
SICI:
0165-2427(20000225)73:2<183:CSAAOC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTATING DAIRY-COWS; FACTOR G-CSF; HUMAN-MONOCYTES; EXPRESSION; CELLS; GENE;
Keywords:
neutrophils; mononuclear cells; cytokine; G-CSF; cDNA; RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Kehrli, ME Iowa State Univ, Dept Microbiol Immunol & Prevent Med, Ames, IA50010 USA Iowa State Univ Ames IA USA 50010 vent Med, Ames, IA 50010 USA
Citazione:
M. Heidari e M.E. Kehrli, "Cloning, sequencing, and analysis of cDNA encoding bovine granulocyte-colony stimulating factor", VET IMMUNOL, 73(2), 2000, pp. 183-191

Abstract

Neutrophils play a critical role in defending against bacterial infections. Hematopoietic growth factors are a class of regulatory cytokines that arerequired for stimulation, proliferation, and differentiation of blood cells. Granulocyte colony stimulating factor (G-CSF) is a cytokine that inducesproliferation and maturation of precursor myeloid cells in the bone marrowinto fully differentiated neutrophils. G-CSF also modulates the functionalactivity of mature neutrophils. Treatment with G-CSF significantly enhances neutrophil phagocytic activity and killing of bacteria and fungi. We haveisolated and sequenced a cDNA clone encoding bovine G-CSF (bG-CSF) from anendothelial cell cDNA library using primers designed from ovine G-CSF. Thefull length cDNA is 1460 nucleotides with 585 nucleotides comprising the open reading frame. Sequence analysis shows 95% identity with ovine, 89% with porcine, 85% with human, and 76% with murine G-CSE The deduced G-CSF protein consists of 174 amino acids with 95% identity to ovine, 86% to porcine,81% to human, and 71% to murine, The signal peptide of C-CSF is 21 amino acids long which is nine amino acids shorter than that of human and murine G-CSE RT-PCR analysis shows that neither freshly isolated nor ConA stimulated neutrophils express G-CSF mRNA. Mononuclear cells, however, expressed G-CSF mRNA after 48 h incubation with or without ConA stimulation. Published by Elsevier Science B.V.

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Documento generato il 04/04/20 alle ore 23:59:10