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Titolo:
Rapid and differential losses of in vivo dopamine transporter (DAT) and vesicular monoamine transporter (VMAT2) radioligand binding in MPTP-treated mice
Autore:
Kilbourn, MR; Kuszpit, K; Sherman, P;
Indirizzi:
Univ Michigan, Sch Med, Dept Internal Med, Div Nucl Med, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 iv Nucl Med, Ann Arbor, MI 48109 USA
Titolo Testata:
SYNAPSE
fascicolo: 4, volume: 35, anno: 2000,
pagine: 250 - 255
SICI:
0887-4476(20000315)35:4<250:RADLOI>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRIATAL DOPAMINE; PARKINSONS-DISEASE; IN-VIVO; 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE MPTP; C57BL/6 MICE; MOUSE-BRAIN; RAT; DIHYDROTETRABENAZINE; METHAMPHETAMINE; METABOLISM;
Keywords:
tetrabenazine; methylphenidate; neurodegeneration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Kilbourn, MR Univ Michigan, 3480 Kresge III, Ann Arbor, MI 48105 USA Univ Michigan 3480 Kresge III Ann Arbor MI USA 48105 8105 USA
Citazione:
M.R. Kilbourn et al., "Rapid and differential losses of in vivo dopamine transporter (DAT) and vesicular monoamine transporter (VMAT2) radioligand binding in MPTP-treated mice", SYNAPSE, 35(4), 2000, pp. 250-255

Abstract

The dose- and time-dependent changes of in vivo radioligand binding to theneuronal membrane dopamine transporter (DAT) and vesicular monoamine transporter type 2 (VMAT2) were examined in mouse brain after MPTP( 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) administrations. Regional brain distribution studies were done in male C57BL/6 mice using simultaneous injections ofd-threo-[H-3]methylphenidate (DAT) and (+)-alpha-[C-11]dihydrotetrabenazine (VMAT2). Single (55 mg/kg i.p.) or multiple (4 x 10 mg/kg i.p., I-hour intervals) administration of MPTP caused significant reductions in [3H]methylphenidate and [C-11] dihydrotetrabenazine specific striatal binding, measured 14 days later. The single high dose of MPTP produced greater losses of [C-11]dihydrotetrabenazine binding than did the multiple MPTP dosing regimen. Using the single high dose of R MPTP, changes of in vivo binding of the two radioligands were determined at 1, 3, and 14 days after neurotoxin injection. At 1 day, there are large losses of [H-3] methylphenidate binding (DAT) but no changes in [C-11] dihydrotetrabenazine binding to the VMAT2 site in the striatum. At 3 and 14 days, there were >50% losses of binding of both bot radioligands, but significantly (P < 0.001) greater losses of VMAT2 binding of [11C]dihydrotetrabenazine. These studies indicate that the lossesof the neuronal membrane and vesicular transporters are not always equal, and do not occur in the same time frame? after administration of the neurotoxin MPTP. (C) 2000 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 16:14:37