Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogen-inducible
Autore:
Puhringer, H; Moll, D; Hoffmann-Sommergruber, K; Watillon, B; Katinger, H; Machado, MLD;
Indirizzi:
Univ Agr Sci, Inst Appl Microbiol, A-1190 Vienna, Austria Univ Agr Sci Vienna Austria A-1190 ppl Microbiol, A-1190 Vienna, Austria Univ Vienna, Inst Gen & Expt Pathol, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 & Expt Pathol, A-1090 Vienna, Austria Fac Uni Sci Agron, Unite Biol Mol & Physiol Anim, B-5030 Gembloux, BelgiumFac Uni Sci Agron Gembloux Belgium B-5030 Anim, B-5030 Gembloux, Belgium
Titolo Testata:
PLANT SCIENCE
fascicolo: 1, volume: 152, anno: 2000,
pagine: 35 - 50
SICI:
0168-9452(20000307)152:1<35:TPOAAY>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
BIRCH POLLEN ALLERGEN; SALICYLIC-ACID; RIBONUCLEASE-ACTIVITY; PLANT TRANSFORMATION; PR PROTEINS; FAMILY; EXPRESSION; BET-V-1; ELICITOR; MEMBERS;
Keywords:
apple allergen; Mal d 1; Nicotiana tabacum; PR-10; promoter analysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Puhringer, H Univ Agr Sci, Inst Appl Microbiol, Muthgasse 18, A-1190 Vienna, Austria Univ Agr Sci Muthgasse 18 Vienna Austria A-1190 nna, Austria
Citazione:
H. Puhringer et al., "The promoter of an apple Ypr10 gene, encoding the major allergen Mal d 1, is stress- and pathogen-inducible", PLANT SCI, 152(1), 2000, pp. 35-50

Abstract

Mal d 1 protein, constituting the major apple allergen, was classified as pathogenesis-related protein 10 (PR-10) on the basis of sequence homologies, although its induction by pathogens or stress has not been demonstrated so far. Here we report the promoter activity of a member of the apple Ypr10 gene family and the inducibility of its gene product Mal d 1. The genomic clone and the promoter sequence of Ypr10*a from Malus domestica have been isolated and characterized. For gene regulation studies the promoter was translationally fused to the uidA reporter gene and expressed in stable transformed Nicotiana tabacum. A total of 1.25 kb of 5' regulating sequence turnedout to be necessary to direct strong beta-glucuronidase (GUS) expression. Besides the already known occurrence of Mel d 1 in ripe fruits, Ypr10 geneswere found to be highly expressed only in old leaves, but can be induced in young leaves by multiple stress factors. Application of abiotic stimuli, like salicylic acid and reduced glutathione significantly increased both, Ypr10*a-GUS activity in transgenic tobacco and transcriptional and translational expression of Mal d 1 in young apple leaves. Virus infection of the transformed tobacco plants strongly induced Ypr10*a-GUS transgene expression. After treatment with fungal elicitors a clear increase in GUS activity andMal d 1 expression was observed in young tobacco and apple leaves, respectively. (C) 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/07/18 alle ore 13:25:31