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Titolo:
Protein analysis methods in diagnosis of sicca syndrome
Autore:
Grus, FH; Augustin, AJ;
Indirizzi:
Univ Mainz, Augenklin, D-55101 Mainz, Germany Univ Mainz Mainz Germany D-55101 ainz, Augenklin, D-55101 Mainz, Germany
Titolo Testata:
OPHTHALMOLOGE
fascicolo: 1, volume: 97, anno: 2000,
pagine: 54 - 61
SICI:
0941-293X(200001)97:1<54:PAMIDO>2.0.ZU;2-5
Fonte:
ISI
Lingua:
GER
Soggetto:
LACRIMAL GLAND; KERATOCONJUNCTIVITIS SICCA; AUTOANTIBODY-REPERTOIRES; TEAR; LACTOFERRIN; PREALBUMIN; ASSAY;
Keywords:
sicca syndrome; tear proteins; high-performance liquid chromatography diagnosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Grus, FH Univ Mainz, Augenklin, Langenbeckstr 1, D-55101 Mainz, Germany Univ Mainz Langenbeckstr 1 Mainz Germany D-55101 Mainz, Germany
Citazione:
F.H. Grus e A.J. Augustin, "Protein analysis methods in diagnosis of sicca syndrome", OPHTHALMOLO, 97(1), 2000, pp. 54-61

Abstract

Background:The routine clinical diagnosis of sicca syndrome remains difficult; the results of the standard tests, such as the Schirmer test, tear film break-up time and the rose Bengal test, correlate neither with the courseof the disease nor with one another. Material and method: We introduce two procedures that can be used to differentiate patients with sicca syndrome from healthy individuals on the basisof tear fluid protein patterns. Electrophoretic separation of the tear proteins or measurement by highperformance liquid chromatography (HPLC) was followed by digital image processing and multivariate discriminant analysis. Results: In addition to analysis of tear protein patterns, these methods permit diagnostic classification. Statistical evaluation reveals whether an unidentified sample is to be classified as sicca syndrome or "healthy". Both HPLC analysis and protein electrophoresis detect differences in protein patterns between the tear fluid of healthy individuals and patients with sicca syndrome and point to a diagnosis accordingly. Conclusion: Both of the techniques presented - electrophoresis and HPLC - could potentially be used for diagnostic purposes in the detection of siccasyndrome. The HPLC method of tear protein analysis is more reliable, lendsitself more readily to automation and achieved greater success rates in the experiments we describe; for these reasons it is the preferred approach.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 12:52:42