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Titolo:
DNA double-strand break rejoining in human follicular lymphoma and glioblastoma tumor cells
Autore:
Macann, AMJ; Britten, RA; Poppema, S; Pearcey, R; Rosenberg, E; Allalunis-Turner, MJ; Murray, D;
Indirizzi:
Univ Alberta, Dept Oncol, Edmonton, AB, Canada Univ Alberta Edmonton AB Canada lberta, Dept Oncol, Edmonton, AB, Canada
Titolo Testata:
ONCOLOGY REPORTS
fascicolo: 2, volume: 7, anno: 2000,
pagine: 299 - 303
SICI:
1021-335X(200003/04)7:2<299:DDBRIH>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
INVERSION GEL-ELECTROPHORESIS; ATAXIA-TELANGIECTASIA; IONIZING-RADIATION; CELLULAR-RECOVERY; REPAIR FIDELITY; LINES; RADIOSENSITIVITY; EXTRACTS; INDUCTION; DAMAGE;
Keywords:
DNA double-strand break rejoining; pulsed-field gel electrophoresis; DNA-repair fidelity; plasmid reactivation; follicular lymphomas;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Murray, D Cross Canc Inst, Dept Expt Oncol, 11560 Univ Ave, Edmonton, AB T6G 1Z2, Canada Cross Canc Inst 11560 Univ Ave Edmonton AB Canada T6G 1Z2 Canada
Citazione:
A.M.J. Macann et al., "DNA double-strand break rejoining in human follicular lymphoma and glioblastoma tumor cells", ONCOL REP, 7(2), 2000, pp. 299-303

Abstract

Follicle center cell lymphoma is among the most radioresponsive of human cancers. To assess whether this radioresponsiveness might be a result of a compromised ability of the tumor cells to accomplish the biologically-effective repair of DNA double-strand breaks (DSBs), we have measured i) the extent of the mechanical rejoining of radiation-induced DSBs in biopsy-derived follicle center cell lymphoma cells and ii) the fidelity with which nuclearprotein extracts from these cells rejoin restriction enzyme-induced DSBs. Cell suspensions derived from two lymphoma biopsies, designated FCL1 and FCL2, as well as two established human glioblastoma cell lines, M059J and M059K, were exposed to 30 Gy of gamma-rays and evaluated for their ability to rejoin DSBs using a Southern transfer-pulsed-field gel electrophoresis assay. The fidelity of rejoining of restriction enzyme-induced DSBs was assessed using a cell-free plasmid reactivation assay. Both lymphoma suspensions rejoined DSBs relatively slowly and exhibited a similar phenotype to the known DSB-rejoining deficient M059J line. The level of DSB misrejoining in thecell-free plasmid reactivation assay was also similar in M059J and FCL2 cells and was considerably (similar to 6-fold) higher than in M059K cells. Because of insufficient numbers of cells, we were unable to perform this assay with the FCL1 lymphoma. These limited data suggest that follicle center cell lymphoma cells may be intrinsically deficient in performing the biologically-effective rejoining of DSBs. Such a deficiency might contribute to the radioresponsiveness of this disease and may be exploitable in the development of improved treatment strategies, such as radioimmunotherapy.

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Documento generato il 24/09/20 alle ore 04:32:33