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Titolo:
EGR-1 enhances tumor growth and modulates the effect of the Wilms' tumor 1gene products on tumorigenicity
Autore:
Scharnhorst, V; Menke, AL; Attema, J; Haneveld, JK; Riteco, N; van Steenbrugge, GJ; van der Eb, AJ; Jochemsen, AG;
Indirizzi:
Leiden Univ, Med Ctr, Mol Carcinogenesis Lab, Dept Mol Cell Biol, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands Leiden Univ, Med Ctr, Ctr Biomed Genet, NL-2300 RA Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2300 RA NL-2300 RA Leiden, Netherlands Erasmus Univ, Dept Expt Urol, NL-3000 DR Rotterdam, Netherlands Erasmus Univ Rotterdam Netherlands NL-3000 DR DR Rotterdam, Netherlands
Titolo Testata:
ONCOGENE
fascicolo: 6, volume: 19, anno: 2000,
pagine: 791 - 800
SICI:
0950-9232(20000210)19:6<791:EETGAM>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-BINDING SPECIFICITY; ZINC FINGER PROTEIN; RAT-KIDNEY-CELLS; WT1 GENE; TRANSFORMING GROWTH-FACTOR-BETA-1; SUPPRESSOR GENE; PROTOONCOGENE TRANSCRIPTION; 3T3 CELLS; EXPRESSION; P53;
Keywords:
Wilms' tumor 1 gene products; early growth response 1 gene product; tumorigenesis; tumor suppression; Wilms' tumor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Jochemsen, AG Leiden Univ, Med Ctr, Mol Carcinogenesis Lab, Dept Mol Cell Biol, POb 9503, NL-2300 RA Leiden, Netherlands Leiden Univ POb 9503 LeidenNetherlands NL-2300 RA herlands
Citazione:
V. Scharnhorst et al., "EGR-1 enhances tumor growth and modulates the effect of the Wilms' tumor 1gene products on tumorigenicity", ONCOGENE, 19(6), 2000, pp. 791-800

Abstract

The Wilms' tumor 1 gene (WT1) encodes a transcription factor of the zinc-finger family and is homozygously mutated or deleted in a subset of Wilms' tumors. Through alternative mRNA splicing, the gene is expressed as four main polypeptides that differ by a stretch of 17 amino acids just N-terminal of the four zinc-fingers and three amino acids between zinc fingers 3 and 4,We have previously shown that expression of the WT1(-/-) isoform, lacking both inserts, increases the tumor growth rate of the adenovirus-transformedbaby rat kidney (AdBRK) cell line 7C3H2, whereas expression of the WT1(-/+) isoform, lacking the 17aa insert, strongly suppresses the tumorigenic phenotype. In the present study we show that expression of these splice variants does not affect the tumorigenic potential of the similar AdBRK cell line, 7C1T1, In contrast to the 7C3H2 cell line, this AdBRK cell line expresseshigh endogenous levels of EGR-1 (early growth response-1) protein, a transcription factor structurally related to WT1, Ectopic expression of EGR-1 inthe 7C3H2 AdBRK cells significantly increases their in vivo growth rate and nullifies the tumor suppressor activity of the WT1(-/+) protein. Furthermore, we find that EGR-1 levels are elevated in some Wilms' tumors. These data are the first to show that EGR-1 overexpression causes enhanced tumor growth and that WT1 and EGR-1 exert antagonizing effects on growth regulationin baby rat kidney cells, which might reflect the situation in some Wilms'tumors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:33:36