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Titolo:
Sedimentation equilibrium analysis of recombinant mouse FcRn with murine IgG1
Autore:
Schuck, P; Radu, CG; Ward, ES;
Indirizzi:
Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Med Ctr, Ctr Immunol, Dallas, TX 75235 USA Univ Texas, SW Med Ctr, Ctr Canc Immunobiol, Dallas, TX 75235 USA Univ Texas Dallas TX USA 75235 Ctr Canc Immunobiol, Dallas, TX 75235 USA NIH, Mol Interact Resource ORS, Bioengn & Phys Sci Program, Bethesda, MD 20892 USA NIH Bethesda MD USA 20892 engn & Phys Sci Program, Bethesda, MD 20892 USA
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 15-16, volume: 36, anno: 1999,
pagine: 1117 - 1125
SICI:
0161-5890(199910/11)36:15-16<1117:SEAORM>2.0.ZU;2-
Fonte:
ISI
Lingua:
ENG
Soggetto:
I-RELATED RECEPTOR; BINDING; RAT; CATABOLISM; COMPLEX; MICE; AFFINITY; IGE; STOICHIOMETRY; LOCALIZATION;
Keywords:
neonatal Fc receptor; sedimentation equilibrium; murine IgG1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Ward, ES Univ Texas, SW Med Ctr, Ctr Immunol, 5323 Harry Hines Blvd, Dallas, TX 75235 USA Univ Texas 5323 Harry Hines Blvd Dallas TX USA 75235 TX 75235 USA
Citazione:
P. Schuck et al., "Sedimentation equilibrium analysis of recombinant mouse FcRn with murine IgG1", MOL IMMUNOL, 36(15-16), 1999, pp. 1117-1125

Abstract

The interaction of mouse IgG1 or IgG1-derived Fc fragment with recombinant, insect cell expressed mouse FcRn has been analyzed using sedimentation equilibrium. This results in a model for the interaction in which the two binding sites for FcRn on Fc or IgG1 have significantly different affinities with macroscopic binding constants of <130 nM and 6 mu M. This data indicates the formation of an asymmetric FcRn:Fe (or IgG1):FcRn complex which is consistent with earlier suggestions that for this form of recombinant FcRn, binding to IgG1 or Fe does not result in a symmetric 2:1 complex in which both binding sites are equivalent. (C) 2000 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:43:26