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Titolo:
Protein-protein interaction of the Ro-ribonucleoprotein particle using multiple antigenic peptides
Autore:
Scofield, RH; Kurien, BT; Zhang, F; Mehta, P; Kaufman, K; Gross, T; Bachmann, M; Gordon, T; Harley, JB;
Indirizzi:
Oklahoma Med Res Fdn, Arthrit & Immunol Program, Oklahoma City, OK 73104 USA Oklahoma Med Res Fdn Oklahoma City OK USA 73104 lahoma City, OK 73104 USA Univ Oklahoma, Hlth Sci Ctr, Dept Med, Oklahoma City, OK USA Univ Oklahoma Oklahoma City OK USA Ctr, Dept Med, Oklahoma City, OK USA Oklahoma Med Res Fdn, Cardiovasc Biol Program, Oklahoma City, OK 73104 USAOklahoma Med Res Fdn Oklahoma City OK USA 73104 lahoma City, OK 73104 USA Dept Vet Affairs Med Ctr, Oklahoma City, OK USA Dept Vet Affairs Med Ctr Oklahoma City OK USA Ctr, Oklahoma City, OK USA WK Warren Med Res Inst, Oklahoma City, OK USA WK Warren Med Res Inst Oklahoma City OK USA Inst, Oklahoma City, OK USA Flinders Med Ctr, Dept Clin Immunol, Bedford Pk, SA, Australia Flinders Med Ctr Bedford Pk SA Australia unol, Bedford Pk, SA, Australia
Titolo Testata:
MOLECULAR IMMUNOLOGY
fascicolo: 15-16, volume: 36, anno: 1999,
pagine: 1093 - 1106
SICI:
0161-5890(199910/11)36:15-16<1093:PIOTRP>2.0.ZU;2-
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; LA SS-B; 5S RIBOSOMAL-RNA; ANTI-RO; AUTOANTIGEN; AUTOANTIBODIES; AUTOIMMUNITY; BINDING; INVITRO; SURFACE;
Keywords:
lupus; autoimmunity; peptides; ribonucleoproteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Scofield, RH Oklahoma Med Res Fdn, Arthrit & Immunol Program, 825 NE 13th St, Oklahoma City, OK 73104 USA Oklahoma Med Res Fdn 825 NE 13th St Oklahoma City OK USA 73104
Citazione:
R.H. Scofield et al., "Protein-protein interaction of the Ro-ribonucleoprotein particle using multiple antigenic peptides", MOL IMMUNOL, 36(15-16), 1999, pp. 1093-1106

Abstract

Protein-protein interactions play a significant role in maintaining the structural and functional integrity of the cell. We used multiple antigen peptides (MAPs) to analyze such interactions within the Ro (or SSA) ribonucleoprotein complex. Our data showed that 60 kD Ro and La colocalize in the nucleus of the cell. Previous data have indicated that 60 kD Po and La co-exist via interactions with the hYRNAs, We were interested to see whether 60 kDRo and La interact with each other through protein-protein interactions. MAPs were produced with sequences derived from the autoepitopes of 60 kD Ro,When used in agarose immunodiffusion certain MAPs formed precipitin lines specifically with Ro and La antigens. Used in affinity chromatography the Ro MAPs purified the Ro ribonucleoprotein particle from lymphocyte extract. Solid phase Immunoassay and surface plasmon resonance (SPR) confirmed the observations obtained with agarose diffusion. Using SPR, kinetic analyses gave an apparent affinity constant of about 1 x 10(7) M-1 for Ro-MAP-60 kD Rointeractions. The autoantigens Ro and La are specific targets in autoimmune diseases, particularly systemic lupus erythematosus (SLE) and Sjogren's syndrome, and are known to exist together as a complex with hYRNAs. The present data indicate that there are protein-protein interactions between Ro and La. Published by Elsevier Science Ltd.

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Documento generato il 01/12/20 alle ore 22:53:42