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Titolo:
2-Chloro-2 '-deoxyadenosine induces apoptosis through the Fas/Fas ligand pathway in human leukemia cell line MOLT-4
Autore:
Nomura, Y; Inanami, O; Takahashi, K; Matsuda, A; Kuwabara, M;
Indirizzi:
Hokkaido Univ, Grad Sch Vet Med, Radiat Biol Lab, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan Hokkaido Univ, Grad Sch Pharmaceut Sci, Med Chem Lab, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan
Titolo Testata:
LEUKEMIA
fascicolo: 2, volume: 14, anno: 2000,
pagine: 299 - 306
SICI:
0887-6924(200002)14:2<299:2'IATT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHRONIC LYMPHOCYTIC-LEUKEMIA; DRUG-INDUCED APOPTOSIS; DOUBLE-STRAND BREAKS; CYTOCHROME-C; PHOSPHATIDYLSERINE EXPOSURE; DEOXYCYTIDINE KINASE; T-LYMPHOCYTES; FAS LIGAND; ANNEXIN-V; 2-CHLORODEOXYADENOSINE;
Keywords:
apoptosis; caspase; 2-chloro-2 '-deoxyadenosine; Fas; MOLT-4;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Kuwabara, M Hokkaido Univ, Grad Sch Vet Med, Radiat Biol Lab, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 o 0600818, Japan
Citazione:
Y. Nomura et al., "2-Chloro-2 '-deoxyadenosine induces apoptosis through the Fas/Fas ligand pathway in human leukemia cell line MOLT-4", LEUKEMIA, 14(2), 2000, pp. 299-306

Abstract

The mechanism of apoptosis induced by 2-chloro-2'-deoxyadenosine (2CdA) inhuman leukemia cell line MOLT-4 was investigated. 2CdA induced increases of 3'-OH ends of genomic DNA, ladder-like DNA fragmentation and phosphatidylserine translocation to the outer membrane, which are apoptotic characteristics. These apoptotic phenomena induced by 2CdA were inhibited by cycloheximide (CHX; a protein synthesis inhibitor), deoxycytidine (dC; a substrate of deoxycytidine kinase), acetyl Ile-Glu-Thr-Asp aldehyde (Ac-IETD-CHO; a caspase-8 inhibitor) and acetyl Asp-Glu-Val-Asp aldehyde (Ac-DEVD-CHO; a caspase-3 inhibitor). The protein synthesis-dependent expression of Fas and Fasligand (Fas-L) was detected by treatment with 2CdA. The proteolytic processing of procaspases-8 and -3 to produce active fragments, caspases-8 (p18) and -3 (p17), respectively, was observed after treatment with 2CdA, and suppressed by cycloheximide. Increases in the activities of caspases-8 and -3 were observed after 2CdA treatment. Their activation was also dependent on protein synthesis. These results indicated that 2CdA-induced apoptosis was triggered by phosphorylation of 2CdA followed by the protein synthesis-dependent expression of Fas and Fas-L and activation of caspases-8 and -3.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:50:57