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Titolo:
Computer-assisted behavioral assessment of pavlovian fear conditioning in mice
Autore:
Anagnostaras, SG; Josselyn, SA; Frankland, PW; Silva, AJ;
Indirizzi:
Univ Calif Los Angeles, Brain Res Inst, Dept Neurobiol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Brain Res Inst, Dept Psychiat, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Brain Res Inst, Dept Psychol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
LEARNING & MEMORY
fascicolo: 1, volume: 7, anno: 2000,
pagine: 58 - 72
SICI:
1072-0502(200001/02)7:1<58:CBAOPF>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONTEXTUAL FEAR; BASOLATERAL AMYGDALA; POTENTIATED STARTLE; DORSAL HIPPOCAMPUS; BLOCKS ACQUISITION; RETROGRADE-AMNESIA; NEUROTOXIC LESIONS; TEMPORAL GRADIENT; RATS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Silva, AJ Univ Calif Los Angeles, Brain Res Inst, Dept Neurobiol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 s, CA 90095 USA
Citazione:
S.G. Anagnostaras et al., "Computer-assisted behavioral assessment of pavlovian fear conditioning in mice", LEARN MEM, 7(1), 2000, pp. 58-72

Abstract

In Pavlovian fear conditioning, a conditional stimulus (CS, usually a tone) is paired with an aversive unconditional stimulus (US, usually a foot shock) in a novel context. After even a single pairing, the animal comes to exhibit a long-lasting fear to the CS add the conditioning context, which canbe measured as freezing, an adaptive defense reaction in mice. Both context and tone conditioning depend on the integrity of the amygdala, and context conditioning further depends on the hippocampus, The reliability and. efficiency of the fear conditioning assay makes it an excellent candidate for the screening of learning and memory deficits in mutant mice. One obstacle is that freezing in mice has been accurately quantified only by human observers, using a tedious method that can be subject to bias. In the present study we generated a simple, high-speed, and highly accurate algorithm that scores freezing of four mice simultaneously using NIH Image on an ordinary Macintosh computer. The algorithm yielded a high correlation and excellent linear fit between computer and human scores across a broad range of conditions. This included the ability to score low pretraining baseline scores andaccurately mimic the effects of two independent variables (shock intensityand test modality) on fear. Because we used. a computer and digital video,we were able to acquire a secondary index of fear, activity suppression, as well as baseline activity scores. Moreover, we measured the unconditionalresponse to shock. These additional measures can enhance the sensitivity of the assay to detect interesting memory phenotypes and control for possible confounds. Thus, this computer-assisted system for measuring behavior during fear conditioning allows for the standardized and carefully controlled assessment of multiple aspects of the fear conditioning experience.

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Documento generato il 28/03/20 alle ore 10:57:01